Microtubules and actin cytoskeleton of potentially pathogenic basidimycetous yeast as target for antifungals

KOPECKÁ, Marie and Miroslav GABRIEL. Microtubules and actin cytoskeleton of potentially pathogenic basidimycetous yeast as target for antifungals. CHEMOTHERAPY. Basel, Switzerland: S. KARGER AG, Basel, 2009, vol. 55, No 4, p. 278-286. ISSN 0009-3157.

Basic information

• Original name: Microtubules and actin cytoskeleton of potentially pathogenic basidimycetous yeast as target for antifungals
• Authors: KOPECKÁ, Marie (203 Czech Republic, guarantor, belonging to the institution) and Miroslav GABRIEL (203 Czech Republic, belonging to the institution).
• Edition: CHEMOTHERAPY, Basel, Switzerland, S. KARGER AG, Basel, 2009, 0009-3157.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL URL
• Impact factor: Impact factor: 2.028
• RIV identification code: RIV/00216224:14110/09:00029343
• Organization unit: Faculty of Medicine
• UT WoS: 000266883400013
• Keywords in English: Yeast;Actin;Microtubules;Paclitaxel;Vincristine;Vinblastine;Methyl benzimidazole2yl carbamate;Thiabendazole; Cytochalasins A B D; Latrunculin A
• Tags: Actin, Cytochalasins A B D, Latrunculin A, Methyl benzimidazole2yl carbamate, Microtubules, paclitaxel, thiabendazole, vinblastine, vincristine, Yeast
• Tags: International impact, Reviewed

Abstract

The cytoskeleton was investigated as a potential target for the inhibition of cell division in Fellomyces fuzhou-ensis related to Cryptococcus neoformans.Vincristine, vinblastine,paclitaxel,methyl benzimidazole-2-yl carbamate (BCM),thiabendazole,cytochalasins A,B,D and latrunculin A were added to cells and investigated.Vincristine, vinblastine, paclitaxel, cytochalasins A,B and D transiently blocked proliferation. BCM disrupted microtubules,inhibited mitosis, but F-actin structures persisted and neck-less conidia originated. Latrunculin disrupted F-actin, cells became spherical,stalks and necks degenerated, microtubules persisted,mitosis,cytokinesis and conidiogenesis were blocked.The combined application of latrunculin and BCM disrupted F-actin and microtubules,inhibited cells became spherical and did not divide.Conclusion: Microtubules and F-actin are effective targets for permanent inhibition of nuclear and cell division and conidiogenesis by BCM and latrunculin A.

Links

• GA310/00/0391, research and development project: Name: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.

Investor: Czech Science Foundation, Cytoskeleton in human pathogenic yeast microorganisms

• GA310/03/1195, research and development project: Name: Cytoskelet jako terčová struktura pro studium účinku antifungálních látek u patogenních kvasinek

Investor: Czech Science Foundation

• GA310/06/0605, research and development project: Name: Lidské patogenní houby : cytoskelet jako potenciální terč inhibice morfogeneze.

Investor: Czech Science Foundation, Human pathogenic fungi: the cytoskeleton as a potential target of inhibition of