HORVÁTH, Radek, Miloš DENDIS, J. SCHLEGELOVA, Filip RŮŽIČKA and J. BENEDÍK. A combined AFLP-multiplex PCR assay for molecular typing of Escherichia coli strains using variable bacterial interspersed mosaic elements. Epidemiology and Infection. Cambridge: Cambridge University Press, 2004, vol. 132, No 1, p. 61-65, 2 pp. ISSN 0950-2688.
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Basic information
Original name A combined AFLP-multiplex PCR assay for molecular typing of Escherichia coli strains using variable bacterial interspersed mosaic elements
Name in Czech Kombinovaná AFLP-multiplex PCR assay pro molekulární typizaci kmenů Escherichia coli
Authors HORVÁTH, Radek (203 Czech Republic, guarantor), Miloš DENDIS (203 Czech Republic), J. SCHLEGELOVA (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic) and J. BENEDÍK (203 Czech Republic).
Edition Epidemiology and Infection, Cambridge, Cambridge University Press, 2004, 0950-2688.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.636
RIV identification code RIV/00216224:14110/04:00035770
Organization unit Faculty of Medicine
UT WoS 000220290200010
Keywords in English AFLP-multiplex PCR; molecular typing; Escherichia coli
Tags AFLP-multiplex PCR, Escherichia coli, molecular typing
Tags International impact, Reviewed
Changed by Changed by: prof. MUDr. Filip Růžička, Ph.D., učo 1100. Changed: 25/6/2009 09:36.
Abstract
The original method for molecular typing of E. coli strains was developed using the polymorphism in chromosomal sequences of bacterial interspersed mosaic elements (BIMEs) detected by Multiplex PCR and analysed by AFLP assay. The applicability of the method in the epidemiology of E. coli was tested on a group of 524 strains of human and veterinary origin. In the studied group 18 different genotypes were detected. Significant differences were found in the frequencies of the genotypes among various groups of strains, suggesting the method could be a promising tool in the epidemiology of E. coli.
Abstract (in Czech)
Tato nová metoda pro molekulární typizaci kmenů E. coli byla vyvinuta na základě polymorfizmu v chromozomálních sekvencích bakteriálních interspersed mosaic elements (BIMEs) detekovaných pomocí Multiplex PCR a AFLP. Využití metody v praxi bylo ověřeno na skupině 524 kmenů humánního i veterinárního původu. V této skupině bylo zjištěno 18 různých genotypů. Mezi jednotlivými genotypy byly zjištěny významné rozdíly ve frekvenci jejich výskytu u jednotlivých skupin. To ukazuje, že metoda může být slibným nástrojem v epidemiologických analýzách E. coli.
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