A case of endocarditis caused by the yeast Pichia fabianii with biofilm production and developed in vitro resistance to azoles in the course of antifungal treatment

HAMAL, Petr, Jiří OSTRANSKY, Miloš DENDIS, Radek HORVÁTH, Filip RŮŽIČKA, Vladimír BUCHTA, Marcela VEJŠOVÁ, Pavel SAUER, Petr HEJNAR and Vladislav RACLAVSKÝ. A case of endocarditis caused by the yeast Pichia fabianii with biofilm production and developed in vitro resistance to azoles in the course of antifungal treatment. Medical Mycology. Oxford, U.K.: Blackwell Science, 2008, vol. 46, No 6, p. 601-605. ISSN 1369-3786.

Basic information

Original name

A case of endocarditis caused by the yeast Pichia fabianii with biofilm production and developed in vitro resistance to azoles in the course of antifungal treatment

Name in Czech

Endokarditida způsobená biofilmpozitivní kvasinkou Pichia fabianii a vznik resistence k azolům v průběhu terapie

Authors

HAMAL, Petr (203 Czech Republic), Jiří OSTRANSKY (203 Czech Republic), Miloš DENDIS (203 Czech Republic), Radek HORVÁTH (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, guarantor), Vladimír BUCHTA (203 Czech Republic), Marcela VEJŠOVÁ (203 Czech Republic), Pavel SAUER (203 Czech Republic), Petr HEJNAR (203 Czech Republic) and Vladislav RACLAVSKÝ (203 Czech Republic)

Edition

Medical Mycology, Oxford, U.K. Blackwell Science, 2008, 1369-3786

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.156

RIV identification code

RIV/00216224:14110/08:00035771

Organization unit

Faculty of Medicine

UT WoS

000258979200011

Keywords in English

INFECTIVE ENDOCARDITIS; HANSENULA-ANOMALA; CROSS-RESISTANCE; CANDIDA; IDENTIFICATION; FUNGEMIA; THERAPY; VORICONAZOLE; CASPOFUNGIN; DIAGNOSIS

Tags

CANDIDA, CASPOFUNGIN, CROSS-RESISTANCE, diagnosis, FUNGEMIA, HANSENULA-ANOMALA, identification, INFECTIVE ENDOCARDITIS, THERAPY, VORICONAZOLE

Tags

International impact, Reviewed

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Abstract

V originále

Pichia fabianii, a yeast rarely causing human infections, was isolated from the blood of a patient with aortic valve endocarditis. The isolates were initially identified biochemically as Candida pelliculosa, but based on direct sequencing of the ITS2 region of rRNA, they were subsequently reidentified as P. fabianii. Antifungal therapy with fluconazole and later with voriconazole led to the development of resistant variants which had high MIC values to both antifungals. Strong biofilm formation by this yeast could also have played a role in the development of its resistance and allowed for its persistence on the infected valve during antifungal therapy. To our knowledge, this is the first published case of endocarditis and the fourth human infection caused by this yeast species.

In Czech

Kvasinka Pichia fabianii, která se vyskytuje jako původce humánních infekcí velmi zřídka, byla izolována z krve pacienta s endokarditidou aortální chlopně. Izolát by původně pomocí biochemických metodidentifikován jako Candida pelliculosa. Později byla kvasinka reidentifikována pomocí sekvenace ITS2 regionu rRNA jako P. fabianii. Antifungální terapie flukonazolem a později i vorikonazolem vedla k rozvoji rezistence k těmto azolovým antimykotikům. V rozvoji rezistence hrála pravděpodobně významnou roli schopnost této kvasinky růst ve formě biofilmu. Tvorba biofilmu také umožnila této kvasince kolonizovat chlopeň a vytvořit zde ložisko, které bylo schopno odolat antifungální terapii.

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Links

NR8365, research and development project

Name: Druhová identifikace patogenních kvasinek založená na analýze teploty tání produktů fingerprintingu