Detailed Information on Publication Record
2009
Effect of Cadmium Chloride on Metallothionein Levels in Carp
KOVAROVA, Jana, Rene KIZEK, Vojtech ADAM, Danka HARUŠTIAKOVÁ, Olga CELECHOVSKA et. al.Basic information
Original name
Effect of Cadmium Chloride on Metallothionein Levels in Carp
Name in Czech
Effect of Cadmium Chloride on Metallothionein Levels in Carp
Authors
KOVAROVA, Jana (203 Czech Republic), Rene KIZEK (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Danka HARUŠTIAKOVÁ (703 Slovakia, guarantor), Olga CELECHOVSKA (203 Czech Republic) and Zdenka SVOBODOVA (203 Czech Republic)
Edition
Sensors, Basel, Switzerland, Molecular Diversity Preservation Interna, 2009, 1424-8220
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10511 Environmental sciences
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.821
RIV identification code
RIV/00216224:14310/09:00035776
Organization unit
Faculty of Science
UT WoS
000267339700044
Keywords (in Czech)
heavy metals; bioaccumulation; Cyprinus carpio
Keywords in English
heavy metals; bioaccumulation; Cyprinus carpio
Tags
International impact, Reviewed
Změněno: 23/2/2010 21:17, RNDr. Danka Haruštiaková, Ph.D.
V originále
Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.
In Czech
Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.
Links
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