A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL
ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY

J 1994

A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY

FISCHER, MB., I. HAUBER, H. EGGENBAUER, Vojtěch THON, E. VOGEL et. al.

Basic information

Original name

A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY

Name in Czech

Porucha časné fáze TCR zprostředkované T buněčné aktivace u pacientů s běžnou variabilní imunodeficiencí

Authors

FISCHER, MB. (40 Austria), I. HAUBER (276 Germany), H. EGGENBAUER (40 Austria), Vojtěch THON (203 Czech Republic, guarantor), E. VOGEL (40 Austria), E. SCHAFFER (40 Austria), J. LOKAJ (203 Czech Republic), J. LITZMAN (203 Czech Republic), HM. WOLF (40 Austria) and JW. MANNHALTER (40 Austria)

Edition

BLOOD, 1994, 0006-4971

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

Russian Federation

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/94:00035804

Organization unit

Faculty of Medicine

UT WoS

A1994PX14600028

Keywords (in Czech)

CVID; TCR; T lymfocyt

Keywords in English

HUMAN B CELLS; PROLIFERATIVE RESPONSES; INTERFERON GAMMA; GENE EXPRESSION; INTERLEUKIN 2; HYPOGAMMAGLOBULINEMIA; IL 2; ANTIGEN; DIFFERENTIATION; ABNORMALITIES

Abstract

ORIG CZ

V originále

Common variable immunodeficiency (CVID) is characterized by an impairment of specific antibody production and a decrease in all or selected Ig isotypes. Abnormalities at the level of the B cells, T cells, and antigen-presenting cells have been described. In the present study, we have focused our attention on T-cell activation in CVID. T cells from 15 of 24 patients failed to respond to recall antigens leg, tetanus toxoid, Escherichia coli), Of these 15 patients, 11 were studied in detail and showed significantly decreased T-cell proliferative responses and/or decreased interleukin-2 and interferon-gamma production on T-cell receptor-mediated stimulation with recall antigens and superantigens (staphylococcal enterotoxins [SEI); however, T-cell response to mitogens (anti-CD3 monoclonal antibody, phytohemagglutinin) was normal. The defect in interleukin-2 and interferon-gamma release on tetanus toroid stimulation could also be documented in purified CD4 T cells of the patients and was present in patients with high and normal CD8 counts alike. Furthermore, patients' T cells failed to mount a significant elevation in free intracellular calcium (Ca++ flux) in response to superantigen, whereas the response to phorbol myristate acetate and ionomycin, bypassing receptor-mediated signaling, was unimpaired. These results indicate a defect in the early phase of T-cell activation after triggering of the T-cell receptor in a significant subgroup of CVID patients.

In Czech

Porucha časné fáze TCR zprostředkované T buněčné aktivace u pacientů s běžnou variabilní imunodeficiencí.

Citovat

FISCHER, MB., I. HAUBER, H. EGGENBAUER, Vojtěch THON, E. VOGEL, E. SCHAFFER, J. LOKAJ, J. LITZMAN, HM. WOLF and JW. MANNHALTER. A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY. BLOOD. 1994, vol. 84, No 12, p. 4234-4241, 7 pp. ISSN 0006-4971.

@article{837110,
   author = {Fischer, MB. and Hauber, I. and Eggenbauer, H. and Thon, Vojtěch and Vogel, E. and Schaffer, E. and Lokaj, J. and Litzman, J. and Wolf, HM. and Mannhalter, JW.},
   article_number = {12},
   keywords = {HUMAN B CELLS; PROLIFERATIVE RESPONSES; INTERFERON GAMMA; GENE EXPRESSION; INTERLEUKIN 2; HYPOGAMMAGLOBULINEMIA; IL 2; ANTIGEN; DIFFERENTIATION; ABNORMALITIES},
   language = {eng},
   issn = {0006-4971},
   journal = {BLOOD},
   title = {A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY},
   volume = {84},
   year = {1994}
}

TY  - JOUR
ID  - 837110
AU  - Fischer, MB. - Hauber, I. - Eggenbauer, H. - Thon, Vojtěch - Vogel, E. - Schaffer, E. - Lokaj, J. - Litzman, J. - Wolf, HM. - Mannhalter, JW.
PY  - 1994
TI  - A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY
JF  - BLOOD
VL  - 84
IS  - 12
SP  - 4234-4241
EP  - 4234-4241
SN  - 00064971
KW  - HUMAN B CELLS
KW  - PROLIFERATIVE RESPONSES
KW  - INTERFERON GAMMA
KW  - GENE EXPRESSION
KW  - INTERLEUKIN 2
KW  - HYPOGAMMAGLOBULINEMIA
KW  - IL 2
KW  - ANTIGEN
KW  - DIFFERENTIATION
KW  - ABNORMALITIES
N2  - Common variable immunodeficiency (CVID) is characterized by an impairment of specific antibody production and a decrease in all or selected Ig isotypes. Abnormalities at the level of the B cells, T cells, and antigen-presenting cells have been described. In the present study, we have focused our attention on T-cell activation in CVID. T cells from 15 of 24 patients failed to respond to recall antigens leg, tetanus toxoid, Escherichia coli), Of these 15 patients, 11 were studied in detail and showed significantly decreased T-cell proliferative responses and/or decreased interleukin-2 and interferon-gamma production on T-cell receptor-mediated stimulation with recall antigens and superantigens (staphylococcal enterotoxins [SEI); however, T-cell response to mitogens (anti-CD3 monoclonal antibody, phytohemagglutinin) was normal. The defect in interleukin-2 and interferon-gamma release on tetanus toroid stimulation could also be documented in purified CD4 T cells of the patients and was present in patients with high and normal CD8 counts alike. Furthermore, patients' T cells failed to mount a significant elevation in free intracellular calcium (Ca++ flux) in response to superantigen, whereas the response to phorbol myristate acetate and ionomycin, bypassing receptor-mediated signaling, was unimpaired. These results indicate a defect in the early phase of T-cell activation after triggering of the T-cell receptor in a significant subgroup of CVID patients.
ER  -

FISCHER, MB., I. HAUBER, H. EGGENBAUER, Vojtěch THON, E. VOGEL, E. SCHAFFER, J. LOKAJ, J. LITZMAN, HM. WOLF and JW. MANNHALTER. A DEFECT IN THE EARLY PHASE OF T-CELL RECEPTOR-MEDIATED T-CELL ACTIVATION IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY. \textit{BLOOD}. 1994, vol.~84, No~12, p.~4234-4241, 7 pp. ISSN~0006-4971.

Detailed Information on Publication Record