Detailed Information on Publication Record
2009
CALCITE MOONMILK AND ITS BACTERIAL ASSOCIATION
KOSINA, Marcel, Andrea TESHIM, Eva NĚMCOVÁ, Ivo SEDLÁČEK, Ondrej ŠEDO et. al.Basic information
Original name
CALCITE MOONMILK AND ITS BACTERIAL ASSOCIATION
Name in Czech
NICKAMÍNEK A JEHO BAKTERIÁLNÍ SPOLEČENSTVÍ
Authors
KOSINA, Marcel (203 Czech Republic, guarantor), Andrea TESHIM (203 Czech Republic), Eva NĚMCOVÁ (203 Czech Republic), Ivo SEDLÁČEK (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic) and Matej LEXA (703 Slovakia)
Edition
17th INTERNATIONAL KARSTOLOGICAL SCHOOL "CLASSICAL KARST" CAVE CLIMATE, 2009
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Slovenia
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/09:00035818
Organization unit
Faculty of Science
Keywords in English
moonmilk; bacteria; Pseudomonas; polyphasic approach; ribotyping; MALDI-MS TOF; SDS-PAGE; 16S rDNA
Tags
Tags
International impact
Změněno: 30/3/2010 18:39, Mgr. Marcel Kosina, Ph.D.
V originále
Caves are considered to be an extreme environment for life. However, closer investigation might reveal that life in caves can be unexpectedly various. Calcite moonmilk, a secondary cave deposit was examined for bacterial component in our study. The origin of this material is still discussed although earlier works showed occurence of microorganisms and the possibility of their influence on formation of moonmilk. Active or passive role of microbiota is still unclear. The aim of our work was to characterize isolated bacterial strains by using a polyphasic taxonomic approach and also find an equivalence of bacterial phenotypic characteristics according to a place of sampling. Moonmilk was sampled in caves of The Moravian Karst, Czech Republic and also in caves in Slovakia. 82 bacterial strains were isolated from the moonmilk samples on four different media. The samples were aerobically cultivated at 10 and 15 degrees of Celsia. The isolated psychrotrophic bacterial strains were characterized by biochemical, physiological tests and whole-cell protein analysis by SDS-PAGE. Selected group of strains was also characterized by ribotyping and MALDI-MS TOF. Partial 16S rRNA gene sequencing was performed on 10 gram-negative strains and followed by sequence alignment analysis with the GenBank BLAST program. Only two isolates represented gram-positive rods and the rest of strains were gram-negative non-fermenting rods. The majority of them were classified as fluorescent pseudomonads with some atypical biochemical properties. Phenotypic characteristics of some strains even showed equivalence among distant places of sampling.
In Czech
Jeskyně jsou považovány za extrémní prostředí pro život. Avšak detailnější výzkumy odhalují, že život v jeskyních může být neočekávaně pestrý. Nickamínek tvořený uhličitanem vápenatým byl podroben mikrobiologickému rozboru. Původ této speleotémy je stále nejasný, ačkoliv dřívější práce potvrzují přítomnost mikroorganismů a naznačují možnost jejich vlivu na utváření nickamínku. Cílem této práce bylo izolovat bakteriální kmeny z nickamínku a následně aplikovat polyfázový taxonomický přístup a najít možné souvislosti mezi místy odběru..
Links
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