Binding of mismatch repair protein MutS to mispaired DNA
adducts of intercalating ruthenium(II) arene complexes

J 2008

Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes

CASTELLANO-CASTILLO, Maria, Hana KOSTRHUNOVA, María Victoria MARINI PALOMEQUE, Jana KAŠPÁRKOVÁ, Peter J. SADLER et. al.

Basic information

Original name

Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes

Name in Czech

Vazba MutS na nezparovane DNA adukty interkalujicich rutheniovych komplexu

Authors

CASTELLANO-CASTILLO, Maria (724 Spain), Hana KOSTRHUNOVA (203 Czech Republic), María Victoria MARINI PALOMEQUE (858 Uruguay), Jana KAŠPÁRKOVÁ (203 Czech Republic), Peter J. SADLER (826 United Kingdom of Great Britain and Northern Ireland), Jean-Marc MALINGE (250 France) and Viktor BRABEC (203 Czech Republic, guarantor)

Edition

Journal of Biological Inorganic Chemistry, Germany, Springer Berlin / Heidelberg, 2008, 0949-8257

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.600

RIV identification code

RIV/00216224:14310/08:00035834

Organization unit

Faculty of Science

UT WoS

000257934500014

Keywords (in Czech)

DNA ; Oprava nezparovanych bazi; MutS; rutheniove areny; interkalace

Keywords in English

DNA ; Mismatch repair ; MutS ; Ruthenium arene ; Intercalation

Abstract

ORIG CZ

V originále

The present study was performed to examine the affinity of Escherichia coli mismatch repair (MMR) protein MutS for DNA damaged by an intercalating compound. We examined the binding properties of this protein with various DNA substrates containing a single centrally located adduct of ruthenium(II) arene complexes [(eta(6)-arene)Ru(II)(en)Cl][PF(6)] [arene is tetrahydroanthracene (THA) or p-cymene (CYM); en is ethylenediamine]. These two complexes were chosen as representatives of two different classes of monofunctional ruthenium(II) arene compounds which differ in DNA-binding modes: one that involves combined coordination to G N7 along with noncovalent, hydrophobic interactions, such as partial arene intercalation (tricyclic-ring Ru-THA), and the other that binds to DNA only via coordination to G N7 and does not interact with double-helical DNA by intercalation (monoring Ru-CYM). Using electrophoretic mobility shift assays, we examined the binding properties of MutS protein with various DNA duplexes (homoduplexes or mismatched duplexes) containing a single centrally located adduct of ruthenium(II) arene compounds. We have shown that presence of the ruthenium(II) arene adducts decreases the affinity of MutS for ruthenated DNA duplexes that either have a regular sequence or contain a mismatch and that intercalation of the arene contributes considerably to this inhibitory effect. Since MutS initiates MMR by recognizing DNA lesions, the results of the present work support the view that DNA damage due to intercalation is removed from DNA by a mechanism(s) other than MMR.

In Czech

Cíl práce byl studovat afinitu proteinu MutS na poškozenou DNA.

Links

LC06030, research and development project

Name: Biomolekulární centrum

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre

Citovat

CASTELLANO-CASTILLO, Maria, Hana KOSTRHUNOVA, María Victoria MARINI PALOMEQUE, Jana KAŠPÁRKOVÁ, Peter J. SADLER, Jean-Marc MALINGE and Viktor BRABEC. Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes. Journal of Biological Inorganic Chemistry. Germany: Springer Berlin / Heidelberg, 2008, vol. 13, No 6, p. 993-999. ISSN 0949-8257.

@article{837281,
   author = {CastellanoandCastillo, Maria and Kostrhunova, Hana and Marini Palomeque, María Victoria and Kašpárková, Jana and Sadler, Peter J. and Malinge, JeanandMarc and Brabec, Viktor},
   article_location = {Germany},
   article_number = {6},
   keywords = {DNA ; Mismatch repair ; MutS ; Ruthenium arene ; Intercalation},
   language = {eng},
   issn = {0949-8257},
   journal = {Journal of Biological Inorganic Chemistry},
   title = {Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes},
   volume = {13},
   year = {2008}
}

TY  - JOUR
ID  - 837281
AU  - Castellano-Castillo, Maria - Kostrhunova, Hana - Marini Palomeque, María Victoria - Kašpárková, Jana - Sadler, Peter J. - Malinge, Jean-Marc - Brabec, Viktor
PY  - 2008
TI  - Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes
JF  - Journal of Biological Inorganic Chemistry
VL  - 13
IS  - 6
SP  - 993-999
EP  - 993-999
PB  - Springer Berlin / Heidelberg
SN  - 09498257
KW  - DNA
KW  - Mismatch repair
KW  - MutS
KW  - Ruthenium arene
KW  - Intercalation
N2  - The present study was performed to examine the affinity of Escherichia coli mismatch repair (MMR) protein MutS for DNA damaged by an intercalating compound. We examined the binding properties of this protein with various DNA substrates containing a single centrally located adduct of ruthenium(II) arene complexes [(eta(6)-arene)Ru(II)(en)Cl][PF(6)] [arene is tetrahydroanthracene (THA) or p-cymene (CYM); en is ethylenediamine]. These two complexes were chosen as representatives of two different classes of monofunctional ruthenium(II) arene compounds which differ in DNA-binding modes: one that involves combined coordination to G N7 along with noncovalent, hydrophobic interactions, such as partial arene intercalation (tricyclic-ring Ru-THA), and the other that binds to DNA only via coordination to G N7 and does not interact with double-helical DNA by intercalation (monoring Ru-CYM). Using electrophoretic mobility shift assays, we examined the binding properties of MutS protein with various DNA duplexes (homoduplexes or mismatched duplexes) containing a single centrally located adduct of ruthenium(II) arene compounds. We have shown that presence of the ruthenium(II) arene adducts decreases the affinity of MutS for ruthenated DNA duplexes that either have a regular sequence or contain a mismatch and that intercalation of the arene contributes considerably to this inhibitory effect. Since MutS initiates MMR by recognizing DNA lesions, the results of the present work support the view that DNA damage due to intercalation is removed from DNA by a mechanism(s) other than MMR.
ER  -

CASTELLANO-CASTILLO, Maria, Hana KOSTRHUNOVA, María Victoria MARINI PALOMEQUE, Jana KAŠPÁRKOVÁ, Peter J. SADLER, Jean-Marc MALINGE and Viktor BRABEC. Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes. \textit{Journal of Biological Inorganic Chemistry}. Germany: Springer Berlin / Heidelberg, 2008, vol.~13, No~6, p.~993-999. ISSN~0949-8257.

Detailed Information on Publication Record