Interaction between Rtg2p and Mks1p in the regulation of the
RTG pathway of Saccharomyces cerevisiae.

J 2005

Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.

ŠPÍREK, Mário, JR FERREIRA JÚNIOR and Ronald BUTOW

Basic information

Original name

Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.

Name in Czech

Interakce mezi Rtg2p a Mks1p v regulaci RTG dráhy Saccharomyces cerevisiae

Authors

ŠPÍREK, Mário (703 Slovakia, guarantor), JR FERREIRA JÚNIOR (76 Brazil) and Ronald BUTOW (840 United States of America)

Edition

Gene, Elsevier, 2005, 0378-1119

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.694

RIV identification code

RIV/00216224:14310/05:00035909

Organization unit

Faculty of Science

UT WoS

000231497200002

Keywords (in Czech)

retrogradni regulace, RTG dráha, Rtg2, Mks1, kvasinka

Keywords in English

Retrograde regulation; RTG pathway; Rtg2p; Mks1p; Yeast

Abstract

ORIG CZ

V originále

Retrograde signaling mediates nuclear gene expression in response to changes in the functional state of mitochondria. In budding yeast, retrograde signaling, also termed the RTG pathway, relies on the heterodimeric, basic helix-loop-helix zipper transcription factors, Rtg1p and Rtg3p, for the activation of target gene expression. Activation of the RTG pathway leads to partial dephosphorylation of Rtg3p and its translocation, together with Rtg1p, from the cytoplasm to the nucleus. These processes depend on a positive regulatory factor, Rtg2p, a novel protein with a ATP binding domain similar to that of the Hsp70/actin/sugar kinase superfamily. Four negative regulatory factors, Lst8p, Mks1p, and two redundant 14-3-3 proteins, Bmh1/2p, function between Rtg2p and Rtg1/3p. Alternative interaction between Mks1p and Rtg2p or Bmh1/2p provides a means for regulation of the RTG pathway. When the RTG pathway is on, Mks1p is inactivated by its association with Rtg2p; and when the RTG pathway is off, Mks1p dissociates from Rtg2p and forms a complex with Bmh1/2p, which is the negative regulatory form of Mks1p. Here we show that Rtg2p and Mks1p can interact in the absence of other factors, and is thereby the minimal binary switch for regulation of the RTG pathway. Gel filtration experiments indicate that both Rtg2p and Mks1p exist in high molecular weight complexes. In response to changes in the activity of the RTG pathway, both Rtg2p and Mks1p shift to different sized high molecular weight complexes. Together, our data suggest that dynamic association between Mks1p and Rtg2p in high molecular weight complexes provides a means to regulate the RTG pathway.

In Czech

Experimenty využívajíc gelovou filtraci naznačili, že Mks1 i Rtg2 existují ve vysoko molekulárních komplexech. Velkost techto komplexú reaguje na aktivitu RTG dráhy.

Citovat

ŠPÍREK, Mário, JR FERREIRA JÚNIOR and Ronald BUTOW. Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae. Gene. Elsevier, 2005, vol. 354, jul 18, p. 2-8, 8 pp. ISSN 0378-1119.

@article{837972,
   author = {Špírek, Mário and Ferreira Júnior, JR and Butow, Ronald},
   article_number = {jul 18},
   keywords = {Retrograde regulation; RTG pathway; Rtg2p; Mks1p; Yeast},
   language = {eng},
   issn = {0378-1119},
   journal = {Gene},
   title = {Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-4GFCPV2-2&_user=835458&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=b0248dee1da31fecb053bc32145972b2},
   volume = {354},
   year = {2005}
}

TY  - JOUR
ID  - 837972
AU  - Špírek, Mário - Ferreira Júnior, JR - Butow, Ronald
PY  - 2005
TI  - Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae.
JF  - Gene
VL  - 354
IS  - jul 18
SP  - 2-8
EP  - 2-8
PB  - Elsevier
SN  - 03781119
KW  - Retrograde regulation
KW  - RTG pathway
KW  - Rtg2p
KW  - Mks1p
KW  - Yeast
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-4GFCPV2-2&_user=835458&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=b0248dee1da31fecb053bc32145972b2
N2  - Retrograde signaling mediates nuclear gene expression in response to changes in the functional state of mitochondria. In budding yeast, retrograde signaling, also termed the RTG pathway, relies on the heterodimeric, basic helix-loop-helix zipper transcription factors, Rtg1p and Rtg3p, for the activation of target gene expression. Activation of the RTG pathway leads to partial dephosphorylation of Rtg3p and its translocation, together with Rtg1p, from the cytoplasm to the nucleus. These processes depend on a positive regulatory factor, Rtg2p, a novel protein with a ATP binding domain similar to that of the Hsp70/actin/sugar kinase superfamily. Four negative regulatory factors, Lst8p, Mks1p, and two redundant 14-3-3 proteins, Bmh1/2p, function between Rtg2p and Rtg1/3p. Alternative interaction between Mks1p and Rtg2p or Bmh1/2p provides a means for regulation of the RTG pathway. When the RTG pathway is on, Mks1p is inactivated by its association with Rtg2p; and when the RTG pathway is off, Mks1p dissociates from Rtg2p and forms a complex with Bmh1/2p, which is the negative regulatory form of Mks1p. Here we show that Rtg2p and Mks1p can interact in the absence of other factors, and is thereby the minimal binary switch for regulation of the RTG pathway. Gel filtration experiments indicate that both Rtg2p and Mks1p exist in high molecular weight complexes. In response to changes in the activity of the RTG pathway, both Rtg2p and Mks1p shift to different sized high molecular weight complexes. Together, our data suggest that dynamic association between Mks1p and Rtg2p in high molecular weight complexes provides a means to regulate the RTG pathway.
ER  -

ŠPÍREK, Mário, JR FERREIRA JÚNIOR and Ronald BUTOW. Interaction between Rtg2p and Mks1p in the regulation of the RTG pathway of Saccharomyces cerevisiae. \textit{Gene}. Elsevier, 2005, vol.~354, jul 18, p.~2-8, 8 pp. ISSN~0378-1119.

Detailed Information on Publication Record