Study of Interactions between Metallothionein and Cisplatin by
using Differential Pulse Voltammetry Brdicka's reaction and
Quartz Crystal Microbalance

J 2009

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

HÚSKA, Dalibor, Ivo FABRIK, Jiří BALOUN, Vojtěch ADAM, Michal MASAŘÍK et. al.

Basic information

Original name

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

Name in Czech

Studium interakcí mezi metalothioneinem a cisplatinou pomocí DPV Brdičkovy reakce a křemenných krystalových mikrovah

Authors

HÚSKA, Dalibor (203 Czech Republic), Ivo FABRIK (203 Czech Republic), Jiří BALOUN (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Michal MASAŘÍK (203 Czech Republic, guarantor), Jaromír HUBÁLEK (203 Czech Republic), Anna VAŠKŮ (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic), Aleš HORNA (203 Czech Republic), Ladislav ZEMAN (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

Sensors, Basel, Switzerland, MDPI, 2009, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.821

RIV identification code

RIV/00216224:14110/09:00036008

Organization unit

Faculty of Medicine

UT WoS

000264572700005

Keywords (in Czech)

Rakovina; Metalothionein; Interakce protein-léčivo; Voltametrie; Brdičkova reakce; Křemenné krystalové mikrováhy

Keywords in English

Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance

Abstract

ORIG CZ

V originále

The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.

In Czech

V této práci byla studována interakce mezi metalothioneinem (MT) a cisplatinou pomocí elektrochemických metodik - DPV, Brdičkova reakce. Signály odpovědné za komplex MT-cisplatina se objevuje v okolí -0.9 V. Tvorba komplexu MT-cisplatina je na čase závislá a byla sledována pomocí křemenných krystalových mikrovah (QCM). Po 20 sekundách jsme mohli pozorovat již zřetelnou změnu, která odpovídá tvorbě komplexu. Navíc jsme stanovovali tento komplex v krvi potkanů, kteří byli léčeni cisplatinou.

Citovat

HÚSKA, Dalibor, Ivo FABRIK, Jiří BALOUN, Vojtěch ADAM, Michal MASAŘÍK, Jaromír HUBÁLEK, Anna VAŠKŮ, Libuše TRNKOVÁ, Aleš HORNA, Ladislav ZEMAN and René KIZEK. Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance. Sensors. Basel, Switzerland: MDPI, 2009, vol. 9, No 3, p. 1355-1369. ISSN 1424-8220.

@article{838425,
   author = {Húska, Dalibor and Fabrik, Ivo and Baloun, Jiří and Adam, Vojtěch and Masařík, Michal and Hubálek, Jaromír and Vašků, Anna and Trnková, Libuše and Horna, Aleš and Zeman, Ladislav and Kizek, René},
   article_location = {Basel, Switzerland},
   article_number = {3},
   keywords = {Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance},
   volume = {9},
   year = {2009}
}

TY  - JOUR
ID  - 838425
AU  - Húska, Dalibor - Fabrik, Ivo - Baloun, Jiří - Adam, Vojtěch - Masařík, Michal - Hubálek, Jaromír - Vašků, Anna - Trnková, Libuše - Horna, Aleš - Zeman, Ladislav - Kizek, René
PY  - 2009
TI  - Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance
JF  - Sensors
VL  - 9
IS  - 3
SP  - 1355-1369
EP  - 1355-1369
PB  - MDPI
SN  - 14248220
KW  - Cancer
KW  - Metallothionein
KW  - Cisplatin
KW  - Protein-Drug Interaction
KW  - Voltammetry
KW  - Brdicka's reaction
KW  - Quartz Crystal Microbalance
N2  - The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.
ER  -

HÚSKA, Dalibor, Ivo FABRIK, Jiří BALOUN, Vojtěch ADAM, Michal MASAŘÍK, Jaromír HUBÁLEK, Anna VAŠKŮ, Libuše TRNKOVÁ, Aleš HORNA, Ladislav ZEMAN and René KIZEK. Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance. \textit{Sensors}. Basel, Switzerland: MDPI, 2009, vol.~9, No~3, p.~1355-1369. ISSN~1424-8220.

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