ŽALOUDÍKOVÁ, Barbora, Martin KELBL, Libor PAŠA and Tomáš FREIBERGER. Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection. Journal of Medical Microbiology. 2009, vol. 58, No 6, 3 pp. ISSN 0022-2615.
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Basic information
Original name Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection
Name in Czech Genotypové versus fenotypové metody detekce prostetické kloubní infekce způsobené Listeria monocytogenes.
Authors ŽALOUDÍKOVÁ, Barbora (203 Czech Republic), Martin KELBL (203 Czech Republic), Libor PAŠA (203 Czech Republic) and Tomáš FREIBERGER (203 Czech Republic, guarantor).
Edition Journal of Medical Microbiology, 2009, 0022-2615.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30300 3.3 Health sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.272
RIV identification code RIV/00216224:14110/09:00040066
Organization unit Faculty of Medicine
UT WoS 000266615900019
Keywords in English PCR; 16S rRNA; Listeria monocytogenes; prosthetic joint infection
Tags 16S rRNA, Listeria monocytogenes, PCR, prosthetic joint infection
Changed by Changed by: prof. MUDr. Tomáš Freiberger, Ph.D., učo 24036. Changed: 7/9/2010 08:46.
Abstract
An uncommon case of a severe prosthetic joint infection (PJI) in a 71-year-old immunocompetent woman is presented. Listeria monocytogenes was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative in the first sample and streptococci of a non-GAS, non-GBS type were detected in the second one. This report demonstrates that the phenotypic approach may lead to misidentification of L. monocytogenes in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected microorganism is present or the sample is collected during antibiotic treatment.
Abstract (in Czech)
V práci je popsán a diskutován případ diskrepantního výsledku molekulárně genetické a klasické kultivační detekce patogena u pacientky s prostetickou kloubní infekcí.
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