ŽALOUDÍKOVÁ, Barbora, Martin KELBL, Libor PAŠA and Tomáš FREIBERGER. Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection. Journal of Medical Microbiology. 2009, vol. 58, No 6, 3 pp. ISSN 0022-2615. |
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@article{838844, author = {Žaloudíková, Barbora and Kelbl, Martin and Paša, Libor and Freiberger, Tomáš}, article_number = {6}, keywords = {PCR; 16S rRNA; Listeria monocytogenes; prosthetic joint infection}, language = {eng}, issn = {0022-2615}, journal = {Journal of Medical Microbiology}, title = {Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection}, volume = {58}, year = {2009} }
TY - JOUR ID - 838844 AU - Žaloudíková, Barbora - Kelbl, Martin - Paša, Libor - Freiberger, Tomáš PY - 2009 TI - Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection JF - Journal of Medical Microbiology VL - 58 IS - 6 SN - 00222615 KW - PCR KW - 16S rRNA KW - Listeria monocytogenes KW - prosthetic joint infection N2 - An uncommon case of a severe prosthetic joint infection (PJI) in a 71-year-old immunocompetent woman is presented. Listeria monocytogenes was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative in the first sample and streptococci of a non-GAS, non-GBS type were detected in the second one. This report demonstrates that the phenotypic approach may lead to misidentification of L. monocytogenes in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected microorganism is present or the sample is collected during antibiotic treatment. ER -
ŽALOUDÍKOVÁ, Barbora, Martin KELBL, Libor PAŠA and Tomáš FREIBERGER. Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection. \textit{Journal of Medical Microbiology}. 2009, vol.~58, No~6, 3 pp. ISSN~0022-2615.
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