Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection

ŽALOUDÍKOVÁ, Barbora, Martin KELBL, Libor PAŠA and Tomáš FREIBERGER. Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection. Journal of Medical Microbiology. 2009, vol. 58, No 6, 3 pp. ISSN 0022-2615.

Basic information

Original name

Genotypic versus phenotypic methods in detection of Listeria monocytogenes prosthetic joint infection

Name in Czech

Genotypové versus fenotypové metody detekce prostetické kloubní infekce způsobené Listeria monocytogenes.

Authors

ŽALOUDÍKOVÁ, Barbora (203 Czech Republic), Martin KELBL (203 Czech Republic), Libor PAŠA (203 Czech Republic) and Tomáš FREIBERGER (203 Czech Republic, guarantor)

Edition

Journal of Medical Microbiology, 2009, 0022-2615

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30300 3.3 Health sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.272

RIV identification code

RIV/00216224:14110/09:00040066

Organization unit

Faculty of Medicine

UT WoS

000266615900019

Keywords in English

PCR; 16S rRNA; Listeria monocytogenes; prosthetic joint infection

Tags

16S rRNA, Listeria monocytogenes, PCR, prosthetic joint infection

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Abstract

V originále

An uncommon case of a severe prosthetic joint infection (PJI) in a 71-year-old immunocompetent woman is presented. Listeria monocytogenes was identified in two consecutive samples using broad-range PCR and sequencing, whereas cultivation remained negative in the first sample and streptococci of a non-GAS, non-GBS type were detected in the second one. This report demonstrates that the phenotypic approach may lead to misidentification of L. monocytogenes in a routine clinical setting. Molecular methods of pathogen detection might be useful when a rare and/or unexpected microorganism is present or the sample is collected during antibiotic treatment.

In Czech

V práci je popsán a diskutován případ diskrepantního výsledku molekulárně genetické a klasické kultivační detekce patogena u pacientky s prostetickou kloubní infekcí.