| KLUMPLER, Tomáš, Blanka PEKÁROVÁ, Jaromír MAREK, Petra BORKOVCOVÁ, Lubomír JANDA and Jan HEJÁTKO. Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana. Acta Crystallographica Section F. 2009, vol. 2009, F65, p. 478-481. ISSN 1744-3091. |
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@article{839508,
author = {Klumpler, Tomáš and Pekárová, Blanka and Marek, Jaromír and Borkovcová, Petra and Janda, Lubomír and Hejátko, Jan},
article_number = {F65},
keywords = {receiver domains; two-component systems; histidine kinases; cytokinins; phosphorelay; CKI1},
language = {eng},
issn = {1744-3091},
journal = {Acta Crystallographica Section F},
title = {Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana.},
volume = {2009},
year = {2009}
}
TY - JOUR
ID - 839508
AU - Klumpler, Tomáš - Pekárová, Blanka - Marek, Jaromír - Borkovcová, Petra - Janda, Lubomír - Hejátko, Jan
PY - 2009
TI - Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana.
JF - Acta Crystallographica Section F
VL - 2009
IS - F65
SP - 478-481
EP - 478-481
SN - 17443091
KW - receiver domains
KW - two-component systems
KW - histidine kinases
KW - cytokinins
KW - phosphorelay
KW - CKI1
N2 - The receiver domain (RD) of a sensor histidine kinase (HK) catalyses the transphosphorylation reaction during the action of HKs in hormonal and abiotic signalling in plants. Crystals of the recombinant RD of the Arabidopsis thaliana HK CYTOKININ-INDEPENDENT1 (CKI1RD) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and glycerol as a cryoprotectant. The crystals diffracted to approximately 2.4 A resolution on beamline BW7B of the DORIS-III storage ring. The diffraction improved significantly after the use of a non-aqueous cryoprotectant. Crystals soaked in Paratone-N diffracted to at least 2.0 A resolution on beamline BW7B and their mosaicity decreased more than tenfold. The crystals belonged to space group C2221, with unit-cell parameters a = 54.46, b = 99.82, c = 79.94 A. Assuming the presence of one molecule of the protein in the asymmetric unit gives a Matthews coefficient VM of 2.33 A3 Da-1. A molecular-replacement solution has been obtained and structure refinement is in progress.
ER -
KLUMPLER, Tomáš, Blanka PEKÁROVÁ, Jaromír MAREK, Petra BORKOVCOVÁ, Lubomír JANDA and Jan HEJÁTKO. Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana. \textit{Acta Crystallographica Section F}. 2009, vol.~2009, F65, p.~478-481. ISSN~1744-3091.
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