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@inproceedings{839526,
author = {Kristová, Petra and Hégrová, Blanka and Farková, Marta and Preisler, Jan and Lubal, Přemysl and Trnková, Libuše},
address = {Brno},
booktitle = {IX. Pracovní setkání fyzikálních chemiků a elektrochemiků},
edition = {2009},
keywords = {purine bases - HPLC - pKa values},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Brno},
isbn = {978-80-7375-309-2},
pages = {49-50},
publisher = {MZLU, Brno},
title = {Purine derivates - separation and characterization by selected methods},
year = {2009}
}
TY - JOUR
ID - 839526
AU - Kristová, Petra - Hégrová, Blanka - Farková, Marta - Preisler, Jan - Lubal, Přemysl - Trnková, Libuše
PY - 2009
TI - Purine derivates - separation and characterization by selected methods
PB - MZLU, Brno
CY - Brno
SN - 9788073753092
KW - purine bases - HPLC - pKa values
N2 - Purine bases represent the basic constituents of DNA, their analysis is useful in the diagnosis of several diseases and metabolite disorders. The related bases, such as 6-aminopurine (6-AP, 2-aminopurine (2-AP) and 2,6-diaminopurine (2,6-DAP) were studies by spectroscopic and RP-HPLC techniques. Studied APs can be separated based on their spectral properties. Elution order related to polarity of molecule, in MF MetOH/30mM phosphate buffer (15:85), was following: 2,6-DAP, 2-AP, 6-AP. The phosphate concentration is not crucial. Experimental pKa values of model APs were calculated from corrected capacity factor dependence of APs on pH. The basicity increasing in order 2-AP<6-AP<2,6-DAP. Studies of basic structures will be used for nucleosides and nucleotides study, where 2-AP and 2,6-DAP can be used as fluorescent labels.
ER -
KRISTOVÁ, Petra, Blanka HÉGROVÁ, Marta FARKOVÁ, Jan PREISLER, Přemysl LUBAL a Libuše TRNKOVÁ. Purine derivates - separation and characterization by selected methods. In \textit{IX. Pracovní setkání fyzikálních chemiků a elektrochemiků}. 2009. vyd. Brno: MZLU, Brno. s.~49-50. ISBN~978-80-7375-309-2. 2009.
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