Continuous electrochemical monitoring of nitric oxide
production in murine macrophage cell line RAW 264.7

J 2009

Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7

PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK et. al.

Basic information

Original name

Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7

Name in Czech

Měření produkce oxidu dusnatého u myších makrofágů RAW 264.7

Authors

PEKAROVÁ, Michaela (703 Slovakia, guarantor), Jana MORAVCOVÁ (203 Czech Republic), Lukáš KUBALA (203 Czech Republic), Milan ČÍŽ (203 Czech Republic), Antonín LOJEK (203 Czech Republic), Čeněk GREGOR (203 Czech Republic) and Jan HRBÁČ (203 Czech Republic)

Edition

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, HEIDELBERG, GERMANY, SPRINGER-VERLAG HEIDELBERG, 2009, 1618-2642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.480

RIV identification code

RIV/00216224:14310/09:00028535

Organization unit

Faculty of Science

UT WoS

000266918600029

Keywords (in Czech)

oxid dusnatý, RAW 264.7, iNOS exprese, senzor pro oxid dusnatý

Keywords in English

Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression

Abstract

ORIG CZ

V originále

In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment.

In Czech

V této studii jsme se zaměřili na jednu z přímých metod stanovení produkce oxidu dusnatého v biologických systémech. Bylo realizováno dlouhodobé a kontinuální elektrochemické měření pomocí modifikovaného mikrosenzoru u makrofágů RAW 264.7.

Links

1QS500040507, research and development project

Name: Tukové složky výživy - modulace jejich účinků a možnosti praktického využití

Citovat

PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR and Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, vol. 394, No 5, p. 1497-1504. ISSN 1618-2642.

@article{839617,
   author = {Pekarová, Michaela and Moravcová, Jana and Kubala, Lukáš and Číž, Milan and Lojek, Antonín and Gregor, Čeněk and Hrbáč, Jan},
   article_location = {HEIDELBERG, GERMANY},
   article_number = {5},
   keywords = {Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression},
   language = {eng},
   issn = {1618-2642},
   journal = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
   title = {Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7},
   volume = {394},
   year = {2009}
}

TY  - JOUR
ID  - 839617
AU  - Pekarová, Michaela - Moravcová, Jana - Kubala, Lukáš - Číž, Milan - Lojek, Antonín - Gregor, Čeněk - Hrbáč, Jan
PY  - 2009
TI  - Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7
JF  - ANALYTICAL AND BIOANALYTICAL CHEMISTRY
VL  - 394
IS  - 5
SP  - 1497-1504
EP  - 1497-1504
PB  - SPRINGER-VERLAG HEIDELBERG
SN  - 16182642
KW  - Nitric oxide
KW  - Macrophages
KW  - RAW 264.7
KW  - Nitric oxide sensor
KW  - Nitrites
KW  - iNOS expression
N2  - In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment.
ER  -

PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR and Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. \textit{ANALYTICAL AND BIOANALYTICAL CHEMISTRY}. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, vol.~394, No~5, p.~1497-1504. ISSN~1618-2642.

Detailed Information on Publication Record