Analysis of significant surface structures of selected parasite
taxa using the scanning eletron microscopy

a 2009

Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy

HODOVÁ, Iveta

Basic information

Original name

Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy

Authors

HODOVÁ, Iveta (203 Czech Republic, guarantor)

Edition

Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia), 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00029407

Organization unit

Faculty of Science

ISBN

978-80-7399-739-7

Keywords in English

SEM;Monogenea;Nematoda

Abstract

V originále

Scanning electron microscopy (SEM) technique was used for research of body surface and surface structures in several taxa of parasites. The fixation methods and preparation of specimens from selected parasite species were optimised. The optimal fixation method was preservation in hot 4% formaldehyde and 3% glutaraldehyde that fixed the parasite body in a straight position and conserved surface structures with the exception of fine cilia. For conservation of ciliate structures the use of the same fixation but at room temperature is more suitable. From the class Monogenea were studied all developmental stages (egg, free-swimming invasive larva oncomiracidium, diporpa, juvenile and adult) of Eudiplozoon nipponicum (Goto, 1891), parasite from the gills of carp. Tegument and sensory structures (type, distribution and function) on body surface were compared among developmental stages. The use of the SEM methodology in small nematodes was found to be necessary for studies of cephalic structures and the caudal end in males, where the study of form and distribution of cloacal and caudal papillae by light microscopy produces inaccurate results. From the class Nematoda parasites from different hosts (from an invertebrate, fishes, a reptile, birds and orang-unan) were studied using SEM.

Links

GA524/07/1610, research and development project

Name: Plasticita fenotypu monogeneí: kombinace molekulárního a morfologického přístupu

Investor: Czech Science Foundation, Phenotypic plasticity of monogenean parasites: a combined molecular and morphological approach

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

Citovat

HODOVÁ, Iveta. Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy. In Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia). 2009. ISBN 978-80-7399-739-7.

@proceedings{840088,
   author = {Hodová, Iveta},
   booktitle = {Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia)},
   keywords = {SEM;Monogenea;Nematoda},
   language = {eng},
   isbn = {978-80-7399-739-7},
   title = {Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy},
   year = {2009}
}

TY  - CONF
ID  - 840088
AU  - Hodová, Iveta
PY  - 2009
TI  - Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy
SN  - 9788073997397
KW  - SEM;Monogenea;Nematoda
N2  - Scanning electron microscopy (SEM) technique was used for research of body surface and surface structures in several taxa of parasites. The fixation methods and preparation of specimens from selected parasite species were optimised. The optimal fixation method was preservation in hot 4% formaldehyde and 3% glutaraldehyde that fixed the parasite body in a straight position and conserved surface structures with the exception of fine cilia. For conservation of ciliate structures the use of the same fixation but at room temperature is more suitable. From the class Monogenea were studied all developmental stages (egg, free-swimming invasive larva oncomiracidium, diporpa, juvenile and adult) of Eudiplozoon nipponicum (Goto, 1891), parasite from the gills of carp. Tegument and sensory structures (type, distribution and function) on body surface were compared among developmental stages. The use of the SEM methodology in small nematodes was found to be necessary for studies of cephalic structures and the caudal end in males, where the study of form and distribution of cloacal and caudal papillae by light microscopy produces inaccurate results. From the class Nematoda parasites from different hosts (from an invertebrate, fishes, a reptile, birds and orang-unan) were studied using SEM.
ER  -

HODOVÁ, Iveta. Analysis of significant surface structures of selected parasite taxa using the scanning eletron microscopy. In \textit{Microscopia 2009Mikroskopia 2009 (25. 3.- 26. 3. 2009 Congress Centre Academia, Stará Lesná, Slovakia)}. 2009. ISBN~978-80-7399-739-7.

Detailed Information on Publication Record