a 2009

Comparison of five PCR methods for characterization of Streptococcus mutans

ŠVEC, Pavel, Dana NOVÁKOVÁ, Martina KUKLETOVÁ and Ivo SEDLÁČEK

Basic information

Original name

Comparison of five PCR methods for characterization of Streptococcus mutans

Name in Czech

Srovnání pěti PCR metod pro typizaci Streptococcus mutans

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor), Dana NOVÁKOVÁ (203 Czech Republic), Martina KUKLETOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)

Edition

3st FEMS Congress of European Microbiologists (FEMS 2009), 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Sweden

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00029411

Organization unit

Faculty of Science

Keywords (in Czech)

Streptococcus mutans; RAPD-PCR; rep-PCR; taxonomie

Keywords in English

Streptococcus mutans; RAPD-PCR; rep-PCR; taxonomy

Tags

Změněno: 10/7/2009 11:22, doc. RNDr. Pavel Švec, Ph.D.

Abstract

ORIG CZ

V originále

Streptococcus mutans attracts great interest of oral clinical microbiologists because it plays a crucial role in the development of dental caries. Development and evaluation of easy to perform, fast and reliable typing methods is important in all clinical studies. RAPD-PCR with M13 primer as well as rep-PCRs with ERIC, REP, BOX and (GTG)5 primers were performed according to the PCR protocols described previously. All S. mutans strains were typeable by M13 primer and revealed 26 fingerprints ranging from 3 000 to 350 bp. Rep-PCR reactions with ERIC, REP and BOX primers revealed only a few very week or none DNA products; in contrast to these results rep-PCR with the (GTG)5 primer showed 24 fingerprints ranging from 3500 to 200 bp. The (GTG)5-PCR method revealed more complex fingerprints than RAPD-PCR. The clustering revealed by both methods was different and showed comparable discriminative power. The study was supported by projects GACR 310/09/0657 and 1M0528.

In Czech

Práce popisuje výsledky typizace kmenů S. mutans s vyuzitím RAPD-PCR s primerem M13 a rep-PCR metod s primery ERIC, REP, BOX a (GTG)5.

Links

GA310/09/0657, research and development project
Name: Molekulární taxonomie grampozitivních bakterií izolovaných ze zubního kazu v dočasné dentici
Investor: Czech Science Foundation, Molecular taxonomy of Gram-positive bacteria isolated from early childhood caries
1M0528, research and development project
Name: Stomatologické výzkumné centrum
Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre