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@inproceedings{840485,
author = {Dejmková, Eva and Pokorná, Marie and Alán, Jan and Wimmerová, Michaela},
address = {Brno},
booktitle = {XIII. Setkání biochemiků a molekulárních biologů},
keywords = {Lectins; Chromobacterium violaceum; changes of binding properties},
language = {eng},
location = {Brno},
isbn = {978-80-210-4830-0},
pages = {25-25},
publisher = {Masarykova univerzita},
title = {Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97},
year = {2009}
}
TY - JOUR
ID - 840485
AU - Dejmková, Eva - Pokorná, Marie - Alán, Jan - Wimmerová, Michaela
PY - 2009
TI - Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97
PB - Masarykova univerzita
CY - Brno
SN - 9788021048300
KW - Lectins
KW - Chromobacterium violaceum
KW - changes of binding properties
N2 - CV-IIL is lectin from human opprotunistic pathogen Chromobacterium violaceum. The aim of this work is a construction of mutant lectins by changing amino acid threonine in the position 97 of carbohydrate binding site. The crystal structure of the CV-IIL lectin shows, that amino acid threonin in position 97 is bound to monosaccharide through one watter molecule. Substitution of this amino acid should lead to changes of sugar binding properties, that could be determinate by the method surface plasmon resonance and isothermal titration microcalorimetry.
ER -
DEJMKOVÁ, Eva, Marie POKORNÁ, Jan ALÁN and Michaela WIMMEROVÁ. Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97. In \textit{XIII. Setkání biochemiků a molekulárních biologů}. Brno: Masarykova univerzita, 2009, p.~25-25. ISBN~978-80-210-4830-0.
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