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@inproceedings{840485, author = {Dejmková, Eva and Pokorná, Marie and Alán, Jan and Wimmerová, Michaela}, address = {Brno}, booktitle = {XIII. Setkání biochemiků a molekulárních biologů}, keywords = {Lectins; Chromobacterium violaceum; changes of binding properties}, language = {eng}, location = {Brno}, isbn = {978-80-210-4830-0}, pages = {25-25}, publisher = {Masarykova univerzita}, title = {Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97}, year = {2009} }
TY - JOUR ID - 840485 AU - Dejmková, Eva - Pokorná, Marie - Alán, Jan - Wimmerová, Michaela PY - 2009 TI - Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97 PB - Masarykova univerzita CY - Brno SN - 9788021048300 KW - Lectins KW - Chromobacterium violaceum KW - changes of binding properties N2 - CV-IIL is lectin from human opprotunistic pathogen Chromobacterium violaceum. The aim of this work is a construction of mutant lectins by changing amino acid threonine in the position 97 of carbohydrate binding site. The crystal structure of the CV-IIL lectin shows, that amino acid threonin in position 97 is bound to monosaccharide through one watter molecule. Substitution of this amino acid should lead to changes of sugar binding properties, that could be determinate by the method surface plasmon resonance and isothermal titration microcalorimetry. ER -
DEJMKOVÁ, Eva, Marie POKORNÁ, Jan ALÁN and Michaela WIMMEROVÁ. Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97. In \textit{XIII. Setkání biochemiků a molekulárních biologů}. Brno: Masarykova univerzita, 2009, p.~25-25. ISBN~978-80-210-4830-0.
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