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@article{840510, author = {Vaňáčová, Štěpánka and Weihong, Y. and Carlton, Jane M. and Johnson, Patricia J.}, article_number = {12}, keywords = {Trichomonas; pre-mRNA splicing; intron; evolution; deep branching eukaryote}, language = {eng}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the U.S.A.}, title = {Spliceosomal introns in a deep-branching eukaryote}, volume = {102}, year = {2005} }
TY - JOUR ID - 840510 AU - Vaňáčová, Štěpánka - Weihong, Y. - Carlton, Jane M. - Johnson, Patricia J. PY - 2005 TI - Spliceosomal introns in a deep-branching eukaryote JF - Proceedings of the National Academy of Sciences of the U.S.A. VL - 102 IS - 12 SP - 4430-4435 EP - 4430-4435 SN - 10916490 KW - Trichomonas KW - pre-mRNA splicing KW - intron KW - evolution KW - deep branching eukaryote N2 - Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3-prime splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote. ER -
VAŇÁČOVÁ, Štěpánka, Y. WEIHONG, Jane M. CARLTON and Patricia J. JOHNSON. Spliceosomal introns in a deep-branching eukaryote. \textit{Proceedings of the National Academy of Sciences of the U.S.A.}. 2005, vol.~102, No~12, p.~4430-4435. ISSN~1091-6490.
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