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@inproceedings{840518,
author = {Fojta, Miroslav and Kostečka, Pavel and Bittová, Miroslava and Bartošík, Martin and Hianik, Tibor and Trnková, Libuše and Paleček, Emil},
address = {Ústí nad Labem},
booktitle = {XXIX. moderní elektrochemické metody - sborník příspěvků},
keywords = {DNA; OsO4 bipy; voltammetry; capillary electrophoresis},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Ústí nad Labem},
isbn = {978-80-86238-39-5},
publisher = {BEST servis},
title = {Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.},
year = {2009}
}
TY - JOUR
ID - 840518
AU - Fojta, Miroslav - Kostečka, Pavel - Bittová, Miroslava - Bartošík, Martin - Hianik, Tibor - Trnková, Libuše - Paleček, Emil
PY - 2009
TI - Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.
PB - BEST servis
CY - Ústí nad Labem
SN - 9788086238395
KW - DNA
KW - OsO4 bipy
KW - voltammetry
KW - capillary electrophoresis
N2 - In this work we used capillary zone electrophoresis (CZE) in combination with square wave voltammetry (SWV) at a pyrolytic graphite electrode (PGE) to study reactivity of different pyrimidine residues, thymine (T), uracil (U), cytosine (C) and 5 methyl cytosine (meC), within a pentanucleotide AAAAY (where Y stands for the pyrimidine residue), towards Os,bipy. Since adenine practically does not react with Os,L, Y residues represent the only reactive centre within the pentamers and their reactivity can thus be easily compared. Both techniques revealed the order of the Y reactivities as follows: T>>MeC~U>C. Hence, our results confirmed (a) highly preferential modification of T among all Ys and (b) increased reactivity of MeC, compared to C that was reported by other authors. However, despite recent papers presenting Os,L modification as useful tool for MeC detection in DNA, the strong modification of T within natural DNA (where T is much more abundant than MeC) makes this approach hardly applicable for real analyses of DNA methylation.
ER -
FOJTA, Miroslav, Pavel KOSTEČKA, Miroslava BITTOVÁ, Martin BARTOŠÍK, Tibor HIANIK, Libuše TRNKOVÁ and Emil PALEČEK. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. In \textit{XXIX. moderní elektrochemické metody - sborník příspěvků}. Ústí nad Labem: BEST servis. 2 pp. ISBN~978-80-86238-39-5. 2009.
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