Electrochemical and capillary zone electrophoresis study of
pyrimidine residues modification by osmium tetroxide,
2,2´-bipyridine. Detection of unpaired and mispaired thymines
in DNA heteroduplexes.

D 2009

Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.

FOJTA, Miroslav, Pavel KOSTEČKA, Miroslava BITTOVÁ, Martin BARTOŠÍK, Tibor HIANIK et. al.

Basic information

Original name

Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.

Name in Czech

Elektrochemická a elektroforetická studie pyrimidinových zbytků modifikovaných OsO4, bipy. Detekce nespárovaných a chybně spárovaných thyminů v heteroduplexech DNA

Authors

FOJTA, Miroslav (203 Czech Republic), Pavel KOSTEČKA (203 Czech Republic), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution), Martin BARTOŠÍK (203 Czech Republic), Tibor HIANIK (703 Slovakia), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution) and Emil PALEČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Ústí nad Labem, XXIX. moderní elektrochemické metody - sborník příspěvků, 2 pp. 2009

Publisher

BEST servis

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14310/09:00036238

Organization unit

Faculty of Science

ISBN

978-80-86238-39-5

Keywords in English

DNA; OsO4 bipy; voltammetry; capillary electrophoresis

Abstract

ORIG CZ

V originále

In this work we used capillary zone electrophoresis (CZE) in combination with square wave voltammetry (SWV) at a pyrolytic graphite electrode (PGE) to study reactivity of different pyrimidine residues, thymine (T), uracil (U), cytosine (C) and 5 methyl cytosine (meC), within a pentanucleotide AAAAY (where Y stands for the pyrimidine residue), towards Os,bipy. Since adenine practically does not react with Os,L, Y residues represent the only reactive centre within the pentamers and their reactivity can thus be easily compared. Both techniques revealed the order of the Y reactivities as follows: T>>MeC~U>C. Hence, our results confirmed (a) highly preferential modification of T among all Ys and (b) increased reactivity of MeC, compared to C that was reported by other authors. However, despite recent papers presenting Os,L modification as useful tool for MeC detection in DNA, the strong modification of T within natural DNA (where T is much more abundant than MeC) makes this approach hardly applicable for real analyses of DNA methylation.

In Czech

V této práci jsme studovali reaktivitu pyrimidinových derivátů (thymin, uracil, 5 methyluracil, cytosin) a OsO4,bipy pomocí voltametrie a kapilární zónové elektroforézy.

Links

LC06035, research and development project

Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

Citovat

FOJTA, Miroslav, Pavel KOSTEČKA, Miroslava BITTOVÁ, Martin BARTOŠÍK, Tibor HIANIK, Libuše TRNKOVÁ and Emil PALEČEK. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. In XXIX. moderní elektrochemické metody - sborník příspěvků. Ústí nad Labem: BEST servis, 2009, 2 pp. ISBN 978-80-86238-39-5.

@inproceedings{840518,
   author = {Fojta, Miroslav and Kostečka, Pavel and Bittová, Miroslava and Bartošík, Martin and Hianik, Tibor and Trnková, Libuše and Paleček, Emil},
   address = {Ústí nad Labem},
   booktitle = {XXIX. moderní elektrochemické metody - sborník příspěvků},
   keywords = {DNA; OsO4 bipy; voltammetry; capillary electrophoresis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Ústí nad Labem},
   isbn = {978-80-86238-39-5},
   publisher = {BEST servis},
   title = {Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.},
   year = {2009}
}

TY  - JOUR
ID  - 840518
AU  - Fojta, Miroslav - Kostečka, Pavel - Bittová, Miroslava - Bartošík, Martin - Hianik, Tibor - Trnková, Libuše - Paleček, Emil
PY  - 2009
TI  - Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.
PB  - BEST servis
CY  - Ústí nad Labem
SN  - 9788086238395
KW  - DNA
KW  - OsO4 bipy
KW  - voltammetry
KW  - capillary electrophoresis
N2  - In this work we used capillary zone electrophoresis (CZE) in combination with square wave voltammetry (SWV) at a pyrolytic graphite electrode (PGE) to study reactivity of different pyrimidine residues, thymine (T), uracil (U), cytosine (C) and 5 methyl cytosine (meC), within a pentanucleotide AAAAY (where Y stands for the pyrimidine residue), towards Os,bipy. Since adenine practically does not react with Os,L, Y residues represent the only reactive centre within the pentamers and their reactivity can thus be easily compared. Both techniques revealed the order of the Y reactivities as follows: T>>MeC~U>C. Hence, our results confirmed (a) highly preferential modification of T among all Ys and (b) increased reactivity of MeC, compared to C that was reported by other authors. However, despite recent papers presenting Os,L modification as useful tool for MeC detection in DNA, the strong modification of T within natural DNA (where T is much more abundant than MeC) makes this approach hardly applicable for real analyses of DNA methylation.
ER  -

FOJTA, Miroslav, Pavel KOSTEČKA, Miroslava BITTOVÁ, Martin BARTOŠÍK, Tibor HIANIK, Libuše TRNKOVÁ and Emil PALEČEK. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. In \textit{XXIX. moderní elektrochemické metody - sborník příspěvků}. Ústí nad Labem: BEST servis, 2009, 2 pp. ISBN~978-80-86238-39-5.

Detailed Information on Publication Record