DVORAK, Jan, Štěpánka VAŇÁČOVÁ, Vladimir HAMPL, Jaroslav FLEGR and Petr HORAK. Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences. Parasitology. UK, 2002, vol. 124, No 3, p. 307-313. ISSN 0031-1820.
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Basic information
Original name Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences
Name in Czech Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences
Authors DVORAK, Jan (203 Czech Republic), Štěpánka VAŇÁČOVÁ (203 Czech Republic, guarantor), Vladimir HAMPL (203 Czech Republic), Jaroslav FLEGR (203 Czech Republic) and Petr HORAK (203 Czech Republic).
Edition Parasitology, UK, 2002, 0031-1820.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.828
RIV identification code RIV/00216224:14310/02:00036245
Organization unit Faculty of Science
UT WoS 000174925000008
Keywords (in Czech) Trichobilharzia; phylogeny; ITS rRNA; Trematodes
Keywords in English Trichobilharzia; phylogeny; ITS rRNA; Trematodes
Tags ITS rRNA, phylogeny, Trematodes, Trichobilharzia
Tags International impact, Reviewed
Changed by Changed by: prof. Mgr. Štěpánka Vaňáčová, Ph.D., učo 105562. Changed: 29/3/2010 16:14.
Abstract
Schistosomes are parasites of considerable medical and veterinary importance and, therefore, all aspects of their biology have been intensively studied. In contrast, our knowledge of species of the largest genus, Trichobilharzia, is insufficient. Because morphological characterization of Trichobilharzia species provides a limited number of criteria for species determination, molecular data are required. In the present paper, we sequenced internal transcribed spacers ITS1 and ITS2, and 5.8S ribosomal RNA (rRNA) genes of 3 European Trichobilharzia species (T. regenti, T. szidati and T. franki). We showed that ITS1 and ITS2 sequences can be used in species identification. Repetitive elements were found in ITS1 of all 3 Trichobilharzia species; their number and length varied depending on the species. Phylogenetic analysis showed that the visceral T. franki is more related to the nasal T. regenti, than to the visceral T. szidati. The newly designed primer, which is specific for T. regenti, might be used as a tool for diagnosis of this potential pathogen.
Abstract (in Czech)
Schistosomes are parasites of considerable medical and veterinary importance and, therefore, all aspects of their biology have been intensively studied. In contrast, our knowledge of species of the largest genus, Trichobilharzia, is insufficient. Because morphological characterization of Trichobilharzia species provides a limited number of criteria for species determination, molecular data are required. In the present paper, we sequenced internal transcribed spacers ITS1 and ITS2, and 5.8S ribosomal RNA (rRNA) genes of 3 European Trichobilharzia species (T. regenti, T. szidati and T. franki). We showed that ITS1 and ITS2 sequences can be used in species identification. Repetitive elements were found in ITS1 of all 3 Trichobilharzia species; their number and length varied depending on the species. Phylogenetic analysis showed that the visceral T. franki is more related to the nasal T. regenti, than to the visceral T. szidati. The newly designed primer, which is specific for T. regenti, might be used as a tool for diagnosis of this potential pathogen.
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