Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences

DVORAK, Jan, Štěpánka VAŇÁČOVÁ, Vladimir HAMPL, Jaroslav FLEGR and Petr HORAK. Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences. Parasitology. UK, 2002, vol. 124, No 3, p. 307-313. ISSN 0031-1820.

Basic information

Original name

Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences

Name in Czech

Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences

Authors

DVORAK, Jan (203 Czech Republic), Štěpánka VAŇÁČOVÁ (203 Czech Republic, guarantor), Vladimir HAMPL (203 Czech Republic), Jaroslav FLEGR (203 Czech Republic) and Petr HORAK (203 Czech Republic)

Edition

Parasitology, UK, 2002, 0031-1820

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Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 1.828

RIV identification code

RIV/00216224:14310/02:00036245

Organization unit

Faculty of Science

UT WoS

000174925000008

Keywords (in Czech)

Trichobilharzia; phylogeny; ITS rRNA; Trematodes

Keywords in English

Trichobilharzia; phylogeny; ITS rRNA; Trematodes

Tags

ITS rRNA, phylogeny, Trematodes, Trichobilharzia

Tags

International impact, Reviewed

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Abstract

V originále

Schistosomes are parasites of considerable medical and veterinary importance and, therefore, all aspects of their biology have been intensively studied. In contrast, our knowledge of species of the largest genus, Trichobilharzia, is insufficient. Because morphological characterization of Trichobilharzia species provides a limited number of criteria for species determination, molecular data are required. In the present paper, we sequenced internal transcribed spacers ITS1 and ITS2, and 5.8S ribosomal RNA (rRNA) genes of 3 European Trichobilharzia species (T. regenti, T. szidati and T. franki). We showed that ITS1 and ITS2 sequences can be used in species identification. Repetitive elements were found in ITS1 of all 3 Trichobilharzia species; their number and length varied depending on the species. Phylogenetic analysis showed that the visceral T. franki is more related to the nasal T. regenti, than to the visceral T. szidati. The newly designed primer, which is specific for T. regenti, might be used as a tool for diagnosis of this potential pathogen.

In Czech

Schistosomes are parasites of considerable medical and veterinary importance and, therefore, all aspects of their biology have been intensively studied. In contrast, our knowledge of species of the largest genus, Trichobilharzia, is insufficient. Because morphological characterization of Trichobilharzia species provides a limited number of criteria for species determination, molecular data are required. In the present paper, we sequenced internal transcribed spacers ITS1 and ITS2, and 5.8S ribosomal RNA (rRNA) genes of 3 European Trichobilharzia species (T. regenti, T. szidati and T. franki). We showed that ITS1 and ITS2 sequences can be used in species identification. Repetitive elements were found in ITS1 of all 3 Trichobilharzia species; their number and length varied depending on the species. Phylogenetic analysis showed that the visceral T. franki is more related to the nasal T. regenti, than to the visceral T. szidati. The newly designed primer, which is specific for T. regenti, might be used as a tool for diagnosis of this potential pathogen.