VAŇÁČOVÁ, Štěpánka, Jan TACHEZY, Elisabetta ULLU and C. TSCHUDI. Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads. Molecular and Biochemical Parasitology. ELSEVIER, 2001, vol. 115, No 2, p. 239-47, 9 pp. ISSN 0166-6851. |
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@article{840532, author = {Vaňáčová, Štěpánka and Tachezy, Jan and Ullu, Elisabetta and Tschudi, C.}, article_number = {2}, keywords = {Trichomonads; RNA-dependent RNA polymerase; RNAP; amanitin}, language = {eng}, issn = {0166-6851}, journal = {Molecular and Biochemical Parasitology}, title = {Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.}, volume = {115}, year = {2001} }
TY - JOUR ID - 840532 AU - Vaňáčová, Štěpánka - Tachezy, Jan - Ullu, Elisabetta - Tschudi, C. PY - 2001 TI - Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads. JF - Molecular and Biochemical Parasitology VL - 115 IS - 2 SP - 239-47 EP - 239-47 PB - ELSEVIER SN - 01666851 KW - Trichomonads KW - RNA-dependent RNA polymerase KW - RNAP KW - amanitin N2 - Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes. ER -
VAŇÁČOVÁ, Štěpánka, Jan TACHEZY, Elisabetta ULLU and C. TSCHUDI. Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads. \textit{Molecular and Biochemical Parasitology}. ELSEVIER, 2001, vol.~115, No~2, p.~239-47, 9 pp. ISSN~0166-6851.
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