VAŇÁČOVÁ, Štěpánka, Jan TACHEZY, Elisabetta ULLU and C. TSCHUDI. Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads. Molecular and Biochemical Parasitology. ELSEVIER, 2001, vol. 115, No 2, p. 239-47, 9 pp. ISSN 0166-6851.
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Basic information
Original name Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.
Name in Czech Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.
Authors VAŇÁČOVÁ, Štěpánka (203 Czech Republic, guarantor), Jan TACHEZY (203 Czech Republic), Elisabetta ULLU (380 Italy) and C. TSCHUDI (756 Switzerland).
Edition Molecular and Biochemical Parasitology, ELSEVIER, 2001, 0166-6851.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.397
RIV identification code RIV/00216224:14310/01:00036247
Organization unit Faculty of Science
UT WoS 000169790600011
Keywords (in Czech) Trichomonads; RNA-dependent RNA polymerase; RNAP; amanitin
Keywords in English Trichomonads; RNA-dependent RNA polymerase; RNAP; amanitin
Tags amanitin, RNA-dependent RNA polymerase, RNAP, Trichomonads
Tags International impact, Reviewed
Changed by Changed by: prof. Mgr. Štěpánka Vaňáčová, Ph.D., učo 105562. Changed: 29/3/2010 16:18.
Abstract
Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes.
Abstract (in Czech)
Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes.
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