HAMPL, Vladimir, Štěpánka VAŇÁČOVÁ, Jaroslav KULDA and Jaroslav FLEGR. Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains. BMC Evolutionary Biology. BioMed Central Ltd: London, 2001, vol. 1, No 11, 10 pp. ISSN 1471-2148.
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Basic information
Original name Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains
Name in Czech Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains
Authors HAMPL, Vladimir (203 Czech Republic), Štěpánka VAŇÁČOVÁ (203 Czech Republic, guarantor), Jaroslav KULDA (203 Czech Republic) and Jaroslav FLEGR (203 Czech Republic).
Edition BMC Evolutionary Biology, BioMed Central Ltd, London, 2001, 1471-2148.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/01:00036250
Organization unit Faculty of Science
UT WoS 000170275500017
Keywords (in Czech) Trichomonads; phylogeny; RAPD; PCR; virulence; metronidazole resistence; ds RNA virus
Keywords in English Trichomonads; phylogeny; RAPD; PCR; virulence; metronidazole resistence; dsRNA virus
Tags dsRNA virus, metronidazole resistence, PCR, phylogeny, RAPD, Trichomonads, VIRULENCE
Tags International impact, Reviewed
Changed by Changed by: prof. Mgr. Štěpánka Vaňáčová, Ph.D., učo 105562. Changed: 29/3/2010 16:18.
Abstract
Despite the medical importance of trichomoniasis, little is known about the genetic relatedness of Trichomonas vaginalis strains with similar biological characteristics. Furthermore, the distribution of endobionts such as mycoplasmas or Trichomonas vaginalis virus (TVV) in the T. vaginalis metapopulation is poorly characterised. RESULTS: We assayed the relationship between 20 strains of T. vaginalis from 8 countries using the Random Amplified Polymorphic DNA (RAPD) analysis with 27 random primers. The genealogical tree was constructed and its bootstrap values were computed using the program FreeTree. Using the permutation tail probability tests we found that the topology of the tree reflected both the pattern of resistance to metronidazole (the major anti-trichomonal drug) (p < 0.01) and the pattern of infection of strains by mycoplasmas (p < 0.05). However, the tree did not reflect pattern of virulence, geographic origin or infection by TVV. Despite low bootstrap support for many branches, the significant clustering of strains with similar drug susceptibility suggests that the tree approaches the true genealogy of strains. The clustering of mycoplasma positive strains may be an experimental artifact, caused by shared RAPD characters which are dependent on the presence of mycoplasma DNA. CONCLUSIONS: Our results confirmed both the suitability of the RAPD technique for genealogical studies in T. vaginalis and previous conclusions on the relatedness of metronidazol resistant strains. However, our studies indicate that testing analysed strains for the presence of endobionts and assessment of the robustness of tree topologies by bootstrap analysis seem to be obligatory steps in such analyses.
Abstract (in Czech)
Despite the medical importance of trichomoniasis, little is known about the genetic relatedness of Trichomonas vaginalis strains with similar biological characteristics. Furthermore, the distribution of endobionts such as mycoplasmas or Trichomonas vaginalis virus (TVV) in the T. vaginalis metapopulation is poorly characterised. RESULTS: We assayed the relationship between 20 strains of T. vaginalis from 8 countries using the Random Amplified Polymorphic DNA (RAPD) analysis with 27 random primers. The genealogical tree was constructed and its bootstrap values were computed using the program FreeTree. Using the permutation tail probability tests we found that the topology of the tree reflected both the pattern of resistance to metronidazole (the major anti-trichomonal drug) (p < 0.01) and the pattern of infection of strains by mycoplasmas (p < 0.05). However, the tree did not reflect pattern of virulence, geographic origin or infection by TVV. Despite low bootstrap support for many branches, the significant clustering of strains with similar drug susceptibility suggests that the tree approaches the true genealogy of strains. The clustering of mycoplasma positive strains may be an experimental artifact, caused by shared RAPD characters which are dependent on the presence of mycoplasma DNA. CONCLUSIONS: Our results confirmed both the suitability of the RAPD technique for genealogical studies in T. vaginalis and previous conclusions on the relatedness of metronidazol resistant strains. However, our studies indicate that testing analysed strains for the presence of endobionts and assessment of the robustness of tree topologies by bootstrap analysis seem to be obligatory steps in such analyses.
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