Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains

HAMPL, Vladimir, Štěpánka VAŇÁČOVÁ, Jaroslav KULDA and Jaroslav FLEGR. Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains. BMC Evolutionary Biology. BioMed Central Ltd: London, 2001, vol. 1, No 11, 10 pp. ISSN 1471-2148.

Basic information

Original name

Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains

Name in Czech

Concordance between genetic relatedness and phenotypic similarities of Trichomonas vaginalis strains

Authors

HAMPL, Vladimir (203 Czech Republic), Štěpánka VAŇÁČOVÁ (203 Czech Republic, guarantor), Jaroslav KULDA (203 Czech Republic) and Jaroslav FLEGR (203 Czech Republic)

Edition

BMC Evolutionary Biology, BioMed Central Ltd, London, 2001, 1471-2148

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00036250

Organization unit

Faculty of Science

UT WoS

000170275500017

Keywords (in Czech)

Trichomonads; phylogeny; RAPD; PCR; virulence; metronidazole resistence; ds RNA virus

Keywords in English

Trichomonads; phylogeny; RAPD; PCR; virulence; metronidazole resistence; dsRNA virus

Tags

dsRNA virus, metronidazole resistence, PCR, phylogeny, RAPD, Trichomonads, VIRULENCE

Tags

International impact, Reviewed

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Abstract

V originále

Despite the medical importance of trichomoniasis, little is known about the genetic relatedness of Trichomonas vaginalis strains with similar biological characteristics. Furthermore, the distribution of endobionts such as mycoplasmas or Trichomonas vaginalis virus (TVV) in the T. vaginalis metapopulation is poorly characterised. RESULTS: We assayed the relationship between 20 strains of T. vaginalis from 8 countries using the Random Amplified Polymorphic DNA (RAPD) analysis with 27 random primers. The genealogical tree was constructed and its bootstrap values were computed using the program FreeTree. Using the permutation tail probability tests we found that the topology of the tree reflected both the pattern of resistance to metronidazole (the major anti-trichomonal drug) (p < 0.01) and the pattern of infection of strains by mycoplasmas (p < 0.05). However, the tree did not reflect pattern of virulence, geographic origin or infection by TVV. Despite low bootstrap support for many branches, the significant clustering of strains with similar drug susceptibility suggests that the tree approaches the true genealogy of strains. The clustering of mycoplasma positive strains may be an experimental artifact, caused by shared RAPD characters which are dependent on the presence of mycoplasma DNA. CONCLUSIONS: Our results confirmed both the suitability of the RAPD technique for genealogical studies in T. vaginalis and previous conclusions on the relatedness of metronidazol resistant strains. However, our studies indicate that testing analysed strains for the presence of endobionts and assessment of the robustness of tree topologies by bootstrap analysis seem to be obligatory steps in such analyses.

In Czech

Despite the medical importance of trichomoniasis, little is known about the genetic relatedness of Trichomonas vaginalis strains with similar biological characteristics. Furthermore, the distribution of endobionts such as mycoplasmas or Trichomonas vaginalis virus (TVV) in the T. vaginalis metapopulation is poorly characterised. RESULTS: We assayed the relationship between 20 strains of T. vaginalis from 8 countries using the Random Amplified Polymorphic DNA (RAPD) analysis with 27 random primers. The genealogical tree was constructed and its bootstrap values were computed using the program FreeTree. Using the permutation tail probability tests we found that the topology of the tree reflected both the pattern of resistance to metronidazole (the major anti-trichomonal drug) (p < 0.01) and the pattern of infection of strains by mycoplasmas (p < 0.05). However, the tree did not reflect pattern of virulence, geographic origin or infection by TVV. Despite low bootstrap support for many branches, the significant clustering of strains with similar drug susceptibility suggests that the tree approaches the true genealogy of strains. The clustering of mycoplasma positive strains may be an experimental artifact, caused by shared RAPD characters which are dependent on the presence of mycoplasma DNA. CONCLUSIONS: Our results confirmed both the suitability of the RAPD technique for genealogical studies in T. vaginalis and previous conclusions on the relatedness of metronidazol resistant strains. However, our studies indicate that testing analysed strains for the presence of endobionts and assessment of the robustness of tree topologies by bootstrap analysis seem to be obligatory steps in such analyses.