Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements

HÚSKA, Dalibor, Vojtěch ADAM, Libuše TRNKOVÁ and René KIZEK. Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements. JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS. 2009, vol. 321, No 10, p. 1474-1477. ISSN 0304-8853.

Basic information

Original name

Dependence of adenine isolation efficiency on the chain length evidenced using paramagnetic particles and voltammetry measurements

Name in Czech

Závislost účinnosti izolace adeninu na délce řetězce s použitím paramagnetických částic a voltametrických měření

Authors

HÚSKA, Dalibor (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution) and René KIZEK (203 Czech Republic, guarantor)

Edition

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2009, 0304-8853

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.204

RIV identification code

RIV/00216224:14310/09:00051184

Organization unit

Faculty of Science

UT WoS

000265278000032

Keywords in English

Paramagnetic particles; Adenine; Adsorptive transfer stripping technique; Square wave voltammetry

Tags

adenine, Adsorptive Transfer Stripping Technique, IK, Paramagnetic particles, rivok, square wave voltammetry

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Abstract

V originále

The main aim of this work was to study the dependence of oligo adenine isolation efficiency on the chain length by using paramagnetic particles covered by homo eoxythymidines ((dT)25) with subsequent detection by adsorptive transfer technique coupled with square wave voltammetry. For this purpose, the oligonucleotides of the length A5, A10, A15, A20, A25, A30, A35, A40 and poly(A) in various concentrations were chosen. We determined that the isolation efficiency defined as "isolated oligonucleotide concentration''/"given oligonucleotide concentration" was about 55% on average. Sequence A25 demonstrated the best binding onto microparticles surface. (c) 2009 Elsevier B.V. All rights reserved.

In Czech

Hlavním cílem této práce bylo studovat závislost účinnosti adeninových oligonukleoticů na délce řetězce s využitím paramagnetických částic, které mají na povrchu thymidinové řetězce. Byly studovány A řetezce o délce 5, 10, 15, 20, 25, 30, 35 a 40 bazí a poly(A. Průměrná účinnost byla 55%. Nejlépe se na částice vázal A25

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Links

LC06035, research and development project

Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu. Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine