Detailed Information on Publication Record
2009
Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
VALIGUROVÁ, Andrea, Veronika MICHALKOVÁ and Břetislav KOUDELABasic information
Original name
Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
Name in Czech
Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
Authors
VALIGUROVÁ, Andrea (703 Slovakia, guarantor, belonging to the institution), Veronika MICHALKOVÁ (703 Slovakia, belonging to the institution) and Břetislav KOUDELA (203 Czech Republic)
Edition
International Journal for Parasitology, Australie, Elsevier Science, 2009, 0020-7519
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Australia
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.819
RIV identification code
RIV/00216224:14310/09:00036268
Organization unit
Faculty of Science
UT WoS
000269115000007
Keywords in English
Epimerite retraction; Epicellular localisation; Eugregarine trophozoite; Actin; Myosin; Gregarina polymorpha
Tags
Tags
International impact, Reviewed
Změněno: 18/3/2011 16:55, doc. RNDr. Andrea Bardůnek Valigurová, Ph.D.
V originále
Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site.
In Czech
Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site.
Links
| LC522, research and development project |
| ||
| MSM0021622416, plan (intention) |
|