VALIGUROVÁ, Andrea, Veronika MICHALKOVÁ and Břetislav KOUDELA. Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact? International Journal for Parasitology. Australie: Elsevier Science, 2009, vol. 2009, No 39, p. 1235-1242. ISSN 0020-7519. |
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@article{840588, author = {Valigurová, Andrea and Michalková, Veronika and Koudela, Břetislav}, article_location = {Australie}, article_number = {39}, keywords = {Epimerite retraction; Epicellular localisation; Eugregarine trophozoite; Actin; Myosin; Gregarina polymorpha}, language = {eng}, issn = {0020-7519}, journal = {International Journal for Parasitology}, title = {Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?}, url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7F-4WB3NFK-2&_user=835458&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1087869357&_rerunOrigin=google&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=140f08d73}, volume = {2009}, year = {2009} }
TY - JOUR ID - 840588 AU - Valigurová, Andrea - Michalková, Veronika - Koudela, Břetislav PY - 2009 TI - Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact? JF - International Journal for Parasitology VL - 2009 IS - 39 SP - 1235-1242 EP - 1235-1242 PB - Elsevier Science SN - 00207519 KW - Epimerite retraction KW - Epicellular localisation KW - Eugregarine trophozoite KW - Actin KW - Myosin KW - Gregarina polymorpha UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7F-4WB3NFK-2&_user=835458&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1087869357&_rerunOrigin=google&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=140f08d73 N2 - Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site. ER -
VALIGUROVÁ, Andrea, Veronika MICHALKOVÁ and Břetislav KOUDELA. Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact? \textit{International Journal for Parasitology}. Australie: Elsevier Science, 2009, vol.~2009, No~39, p.~1235-1242. ISSN~0020-7519.
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