Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?

VALIGUROVÁ, Andrea, Veronika MICHALKOVÁ and Břetislav KOUDELA. Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact? International Journal for Parasitology. Australie: Elsevier Science, 2009, vol. 2009, No 39, p. 1235-1242. ISSN 0020-7519.

Basic information

• Original name: Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
• Name in Czech: Eugregarine trophozoite detachment from the host epithelium via epimerite retraction: Fiction or fact?
• Authors: VALIGUROVÁ, Andrea (703 Slovakia, guarantor, belonging to the institution), Veronika MICHALKOVÁ (703 Slovakia, belonging to the institution) and Břetislav KOUDELA (203 Czech Republic).
• Edition: International Journal for Parasitology, Australie, Elsevier Science, 2009, 0020-7519.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Australia
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.819
• RIV identification code: RIV/00216224:14310/09:00036268
• Organization unit: Faculty of Science
• UT WoS: 000269115000007
• Keywords in English: Epimerite retraction; Epicellular localisation; Eugregarine trophozoite; Actin; Myosin; Gregarina polymorpha
• Tags: Actin, Epicellular localisation, Epimerite retraction, Eugregarine trophozoite, Gregarina polymorpha, myosin
• Tags: International impact, Reviewed

Abstract

Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site.

Abstract (in Czech)

Using electron and fluorescence microscopy, the fine structure of the epimerite with associated structures and the mechanism of trophozoite detachment from the host epithelium were studied in Gregarina polymorpha parasitising the intestine of Tenebrio molitor larvae. The epimerite appears to be a very dynamic structure whose shape dramatically changes depending on whether or not it is embedded into the host epithelium. FITC-phalloidin labelling demonstrated the presence of f-actin in trophozoites along with its accumulation at the epimerite base and in the protomerite apical end. IFA revealed the presence of myosin in the epimeritic cortical zone and in the membrane fusion site area. We propose a new hypothesis on the mechanism of trophozoite detachment from the host epithelium based on epimerite retraction into the protomerite. This is contrary to the commonly accepted hypothesis describing gradual epimerite constriction and separation facilitated by contractility of the membrane fusion site.

Links

• LC522, research and development project: Name: ICHTYOPARAZITOLOGIE - centrum základního výzkumu

Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time