| CHEMANI, Chanez, Anne IMBERTY, Sophie DE BENTZMANN, Maud PIERRE, Michaela WIMMEROVÁ, Benoit P. GUERY and Karine FAURE. Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands. INFECTION AND IMMUNITY. The American Society for Microbiology, 2009, vol. 77, No 5, p. 2065-2075. ISSN 0019-9567. |
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@article{842446,
author = {Chemani, Chanez and Imberty, Anne and de Bentzmann, Sophie and Pierre, Maud and Wimmerová, Michaela and Guery, Benoit P. and Faure, Karine},
article_number = {5},
keywords = {lectin; infection;preudomonas aeruginosa},
language = {cze},
issn = {0019-9567},
journal = {INFECTION AND IMMUNITY},
title = {Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands},
volume = {77},
year = {2009}
}
TY - JOUR
ID - 842446
AU - Chemani, Chanez - Imberty, Anne - de Bentzmann, Sophie - Pierre, Maud - Wimmerová, Michaela - Guery, Benoit P. - Faure, Karine
PY - 2009
TI - Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands
JF - INFECTION AND IMMUNITY
VL - 77
IS - 5
SP - 2065-2075
EP - 2065-2075
PB - The American Society for Microbiology
SN - 00199567
KW - lectin
KW - infection;preudomonas aeruginosa
N2 - Pseudomonas aeruginosa is a frequently encountered pathogen that is involved in acute and chronic lung infections. Lectin-mediated bacterium-cell recognition and adhesion are critical steps in initiating P. aeruginosa pathogenesis. This study was designed to evaluate the contributions of LecA and LecB to the pathogenesis of P. aeruginosa-mediated acute lung injury. Using an in vitro model with A549 cells and an experimental in vivo murine model of acute lung injury, we compared the parental strain to lecA and lecB mutants. The effects of both LecA- and Lec B-specific lectin-inhibiting carbohydrates (a-methyl-galactoside and a-methyl-fucoside, respectively) were evaluated. In vitro, the parental strain was associated with increased cytotoxicity and adhesion on A549 cells compared to the lecA and lecB mutants. In vivo, the P. aeruginosa-induced increase in alveolar barrier permeability was reduced with both mutants. The bacterial burden and dissemination were decreased for both mutants compared with the parental strain. Coadministration of specific lectin inhibitors markedly reduced lung injury and mortality. Our results demonstrate that there is a relationship between lectins and the pathogenicity of P. aeruginosa
ER -
CHEMANI, Chanez, Anne IMBERTY, Sophie DE BENTZMANN, Maud PIERRE, Michaela WIMMEROVÁ, Benoit P. GUERY and Karine FAURE. Role of LecA and LecB Lectins in Pseudomonas aeruginosa-Induced Lung Injury and Effect of Carbohydrate Ligands. \textit{INFECTION AND IMMUNITY}. The American Society for Microbiology, 2009, vol.~77, No~5, p.~2065-2075. ISSN~0019-9567.
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