Elimination Voltammetry as an Analytical Tool for the DNA sensors

TRNKOVÁ, Libuše, František JELEN, Vojtěch ADAM and René KIZEK. Elimination Voltammetry as an Analytical Tool for the DNA sensors. In The 60th Annual Meeting of the International Society of Electrochemistry – book of abstracts. Beijing, China: International Society of Electrochemistry, 2009, 1 pp.

Basic information

• Original name: Elimination Voltammetry as an Analytical Tool for the DNA sensors
• Name in Czech: Eliminační voltametrie jako analytický nástroj pro DNA senzory
• Authors: TRNKOVÁ, Libuše, František JELEN, Vojtěch ADAM and René KIZEK.
• Edition: Beijing, China, The 60th Annual Meeting of the International Society of Electrochemistry – book of abstracts, 1 pp. 2009.
• Publisher: International Society of Electrochemistry

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10405 Electrochemistry
• Country of publisher: China
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords in English: DNA sensor; voltammetry
• Tags: International impact

Abstract

The EVLS can be considered as a mathematical model of the transformation of current-potential curves capable of eliminating some selected current components, while conserving others by means of elimination functions. Its application is very simple. The chosen elimination function needs only two or three voltammetric (LSV or CV) curves measured at different scan rates. For totally adsorbed electroactive species the function eliminating charging and kinetic current components, and conserving the diffusion current component, yields for totally adsorbed electroactive species the specific, sensitive and well developed peak-counterpeak (p-cp) signal. This signal, usually 10-20 times higher than corresponding measured voltammetric peak is successfully employed in the analysis of nucleic acids and short homo- or hetero-deoxyoligonucleotides (ODNs) containing adenine and cytosine. Moreover, the EVLS increased the current sensitivity for A and C resolution and, thus, for the recognition of bases sequences in ODN chains. It has been shown that the EVLS in combination with adsorptive stripping procedure is a promising tool for achieving very good resolution of electrode processes, for qualitative and quantitative analysis of ODNs and their components, as well as for identification of ODN structures.

Abstract (in Czech)

Metoda EVLS je považována za matematický model transformace proudově napěťových křivek, který je schopen eliminovat některé vybrané složky proudu, zatímco ostatní zachovává. EVLS se s úspěchem využívá při analýze směsí oligonukleotidů obsahujících A a C. EVLS ve spojení s adsorptivní rozpouštěcí technikou je velmi dobrý nástroj pro kvalitativní i kvantitativní analýzu oligonukleotidů a jejich složek, i pro identifikaci jejich struktur.

Links

• LC06035, research and development project: Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)