a 2009

Fungicides and insecticides effects on photosynthesis and photoprotective mechanisms in A. thaliana under high light and high temperature stress

LANG, Jaroslav, Miloš BARTÁK, Kristýna VEČEŘOVÁ and Hana CEMPÍRKOVÁ

Basic information

Original name

Fungicides and insecticides effects on photosynthesis and photoprotective mechanisms in A. thaliana under high light and high temperature stress

Name in Czech

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Authors

LANG, Jaroslav (203 Czech Republic, guarantor), Miloš BARTÁK (203 Czech Republic), Kristýna VEČEŘOVÁ (203 Czech Republic) and Hana CEMPÍRKOVÁ (203 Czech Republic)

Edition

7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009, 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14310/09:00028579

Organization unit

Faculty of Science

ISBN

978-80-7375-310-8

Keywords (in Czech)

fotoinhibice, fotoochrana, fotosyntéza, stres, antioxidanty

Keywords in English

photoinhibition; photoprotection; photosynthesis; stress; antioxidants
Změněno: 11/8/2010 10:38, prof. Ing. Miloš Barták, CSc.

Abstract

V originále

In cultivations of the most common experimental plant A. thaliana, chemical control is unevoidable. We invesigated co-effects of fungicides/insecticides and high light/heat treatments. Wild type A. thaliana (Columbia) and LHC-defficient mutants (Lhcb 2-1) were used and treated each 30 d by the following chemicals: (1) Previcur 607SL, (2) Zolone 35EC, (3) a mixture of 0,25% Previcur and 0,2% Zolone, and (4) untreated control. Additionally, the plants were exposed to 2 short-term treatmets: (a) 1000 micromol m-2 s-1 of PAR (high light = HL) for 60 min and (b) combination of HL and 30 oC. Treatment effects on photosynthesis were monitored by a PAM-2000 fluorometer. Before and after the treatments, pigments were extracted from leaf tissue and HPLC determined: contents of chla, chlb, viola-, anthera-, zeaxanthin, lutein, beta-carotene were evaluated by a HPLC (Waters). Results showed that Previcur plus Zolone caused oxidative stress in chloroplasts even in control plants indicaded by increased values of zeaxanthin and DEPS. In Lhcb mutants, Fv/Fm decreased after HL and temperature treatments which was attributed to negative short-term effects in photosystem II. Supported by FRVS 1546/2008.

In Czech

Práce přináší poznatky o společných účincích fungicidů/insekticidů kombinovaných s vysokými hodnotami ozáření a vysokou teplotou na rostliny, respektive jejich fotosyntetický aparát.

Links

KJB601630808, research and development project
Name: Účinky chladu a mrazu na fotosyntézu lišejníkových symbiotických řas ovlivněných ribitolem.
Investor: Academy of Sciences of the Czech Republic, Effects of low and freezing temperatures on photosynthesis in lichen symbiotic algae affected by ribitol