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@proceedings{842885,
author = {Lang, Jaroslav and Barták, Miloš and Večeřová, Kristýna and Cempírková, Hana},
booktitle = {7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009},
keywords = {photoinhibition; photoprotection; photosynthesis; stress; antioxidants},
language = {eng},
isbn = {978-80-7375-310-8},
title = {Fungicides and insecticides effects on photosynthesis and photoprotective mechanisms in A. thaliana under high light and high temperature stress},
url = {http://phd.czweb.org/phd09/},
year = {2009}
}
TY - CONF
ID - 842885
AU - Lang, Jaroslav - Barták, Miloš - Večeřová, Kristýna - Cempírková, Hana
PY - 2009
TI - Fungicides and insecticides effects on photosynthesis and photoprotective mechanisms in A. thaliana under high light and high temperature stress
SN - 9788073753108
KW - photoinhibition
KW - photoprotection
KW - photosynthesis
KW - stress
KW - antioxidants
UR - http://phd.czweb.org/phd09/
N2 - In cultivations of the most common experimental plant A. thaliana, chemical control is unevoidable. We invesigated co-effects of fungicides/insecticides and high light/heat treatments. Wild type A. thaliana (Columbia) and LHC-defficient mutants (Lhcb 2-1) were used and treated each 30 d by the following chemicals: (1) Previcur 607SL, (2) Zolone 35EC, (3) a mixture of 0,25% Previcur and 0,2% Zolone, and (4) untreated control. Additionally, the plants were exposed to 2 short-term treatmets: (a) 1000 micromol m-2 s-1 of PAR (high light = HL) for 60 min and (b) combination of HL and 30 oC. Treatment effects on photosynthesis were monitored by a PAM-2000 fluorometer. Before and after the treatments, pigments were extracted from leaf tissue and HPLC determined: contents of chla, chlb, viola-, anthera-, zeaxanthin, lutein, beta-carotene were evaluated by a HPLC (Waters). Results showed that Previcur plus Zolone caused oxidative stress in chloroplasts even in control plants indicaded by increased values of zeaxanthin and DEPS. In Lhcb mutants, Fv/Fm decreased after HL and temperature treatments which was attributed to negative short-term effects in photosystem II. Supported by FRVS 1546/2008.
ER -
LANG, Jaroslav, Miloš BARTÁK, Kristýna VEČEŘOVÁ and Hana CEMPÍRKOVÁ. Fungicides and insecticides effects on photosynthesis and photoprotective mechanisms in A. thaliana under high light and high temperature stress. In \textit{7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009}. 2009. ISBN~978-80-7375-310-8.
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