ŠTEPIGOVÁ, Jana, Kristýna VEČEŘOVÁ and Miloš BARTÁK. Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress? In 7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009. 2009. ISBN 978-80-7375-310-8.
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Basic information
Original name Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?
Name in Czech Hraje množství světlosběrných komplexů roli v citlivosti rostlin A. thalina ovlivněných DCMU na stres vyokým zářením?
Authors ŠTEPIGOVÁ, Jana (703 Slovakia), Kristýna VEČEŘOVÁ (203 Czech Republic) and Miloš BARTÁK (203 Czech Republic, guarantor).
Edition 7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009, 2009.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14310/09:00029456
Organization unit Faculty of Science
ISBN 978-80-7375-310-8
Keywords (in Czech) fotoinhibice; LHC mutanti; fotosystém II; stres; antioxidanty; fluorometrie
Keywords in English photoinhibition; LHC lacking mutants; photosystem II; stress; chlorophyll fluorescence
Changed by Changed by: prof. Ing. Miloš Barták, CSc., učo 57. Changed: 31/3/2010 14:54.
Abstract
Several fluorometric techniques, such as chlorophyll(Chl) fluorescence imaging, saturation pulse method, fast Chl fluorescence transients (OJIPs) were used to study photoinhibition and recovery in potted plants of A. thaliana. To address the role of light harvesting complexes(LHCs), wild type (wt-Columbia), npq (defective in zeaxanthin deepoxidase), and lhc (40 % reduction in LHCs) mutants were used. Responses to short-term (2000 microE m-2 s-1 PAR for 30 min) and long-term (300 microE m-2 s-1 PAR for 30 min plus PS II blocker DCMU) treatments were measured. In short-term experiment, photoinhibition of PS II was apparent as a decrease in Chl fluorescence in OJIPs, PS II quantum yield, and Fv/Fm ratio. The parameters, however, showed rapid recovery after 180 min in dark indicating high capacity of photoprotective mechanisms. Timecourses of Chl fluorescence parameters differed between wt, npq and lhc plants. In long-term treatment, the rate of DCMU (applied into substrate) uptake and interaction with light differed between wt and lhc plants. The results indicated that LHC-deficient A. thaliana mutants were more sensitive to photoinhibition than wt control. Supported by GACR52206097.
Abstract (in Czech)
Měkolik metod indukované fluorescence chlorofylu bylo využito při studiu projevů fotoinhibice ve fotosystému II A. thaliana, a to rostlin kontrolních (wt), mutantů deficientních zeaxantin deepoxidázou a mutantů s redukovaným množstvím světlosběrných komplexů (LHC).
Links
GA522/06/0979, research and development projectName: Mechanismy odolnosti rostlin proti ROS indukovaným ve fotosyntetickém aparátu během fotoinhibičního stresu
Investor: Czech Science Foundation, Mechanism of plant resistance against ROS induced in photosynthetic apparatus by a photoinhibitory stress
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