a 2009

Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?

ŠTEPIGOVÁ, Jana, Kristýna VEČEŘOVÁ and Miloš BARTÁK

Basic information

Original name

Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?

Name in Czech

Hraje množství světlosběrných komplexů roli v citlivosti rostlin A. thalina ovlivněných DCMU na stres vyokým zářením?

Authors

ŠTEPIGOVÁ, Jana (703 Slovakia), Kristýna VEČEŘOVÁ (203 Czech Republic) and Miloš BARTÁK (203 Czech Republic, guarantor)

Edition

7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009, 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

RIV identification code

RIV/00216224:14310/09:00029456

Organization unit

Faculty of Science

ISBN

978-80-7375-310-8

Keywords (in Czech)

fotoinhibice; LHC mutanti; fotosystém II; stres; antioxidanty; fluorometrie

Keywords in English

photoinhibition; LHC lacking mutants; photosystem II; stress; chlorophyll fluorescence
Změněno: 31/3/2010 14:54, prof. Ing. Miloš Barták, CSc.

Abstract

ORIG CZ

V originále

Several fluorometric techniques, such as chlorophyll(Chl) fluorescence imaging, saturation pulse method, fast Chl fluorescence transients (OJIPs) were used to study photoinhibition and recovery in potted plants of A. thaliana. To address the role of light harvesting complexes(LHCs), wild type (wt-Columbia), npq (defective in zeaxanthin deepoxidase), and lhc (40 % reduction in LHCs) mutants were used. Responses to short-term (2000 microE m-2 s-1 PAR for 30 min) and long-term (300 microE m-2 s-1 PAR for 30 min plus PS II blocker DCMU) treatments were measured. In short-term experiment, photoinhibition of PS II was apparent as a decrease in Chl fluorescence in OJIPs, PS II quantum yield, and Fv/Fm ratio. The parameters, however, showed rapid recovery after 180 min in dark indicating high capacity of photoprotective mechanisms. Timecourses of Chl fluorescence parameters differed between wt, npq and lhc plants. In long-term treatment, the rate of DCMU (applied into substrate) uptake and interaction with light differed between wt and lhc plants. The results indicated that LHC-deficient A. thaliana mutants were more sensitive to photoinhibition than wt control. Supported by GACR52206097.

In Czech

Měkolik metod indukované fluorescence chlorofylu bylo využito při studiu projevů fotoinhibice ve fotosystému II A. thaliana, a to rostlin kontrolních (wt), mutantů deficientních zeaxantin deepoxidázou a mutantů s redukovaným množstvím světlosběrných komplexů (LHC).

Links

GA522/06/0979, research and development project
Name: Mechanismy odolnosti rostlin proti ROS indukovaným ve fotosyntetickém aparátu během fotoinhibičního stresu
Investor: Czech Science Foundation, Mechanism of plant resistance against ROS induced in photosynthetic apparatus by a photoinhibitory stress