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@proceedings{842888,
author = {Štepigová, Jana and Večeřová, Kristýna and Barták, Miloš},
booktitle = {7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009},
keywords = {photoinhibition; LHC lacking mutants; photosystem II; stress; chlorophyll fluorescence},
language = {eng},
isbn = {978-80-7375-310-8},
title = {Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?},
url = {http://phd.czweb.org/phd09/},
year = {2009}
}
TY - CONF
ID - 842888
AU - Štepigová, Jana - Večeřová, Kristýna - Barták, Miloš
PY - 2009
TI - Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress?
SN - 9788073753108
KW - photoinhibition
KW - LHC lacking mutants
KW - photosystem II
KW - stress
KW - chlorophyll fluorescence
UR - http://phd.czweb.org/phd09/
N2 - Several fluorometric techniques, such as chlorophyll(Chl) fluorescence imaging, saturation pulse method, fast Chl fluorescence transients (OJIPs) were used to study photoinhibition and recovery in potted plants of A. thaliana. To address the role of light harvesting complexes(LHCs), wild type (wt-Columbia), npq (defective in zeaxanthin deepoxidase), and lhc (40 % reduction in LHCs) mutants were used. Responses to short-term (2000 microE m-2 s-1 PAR for 30 min) and long-term (300 microE m-2 s-1 PAR for 30 min plus PS II blocker DCMU) treatments were measured. In short-term experiment, photoinhibition of PS II was apparent as a decrease in Chl fluorescence in OJIPs, PS II quantum yield, and Fv/Fm ratio. The parameters, however, showed rapid recovery after 180 min in dark indicating high capacity of photoprotective mechanisms. Timecourses of Chl fluorescence parameters differed between wt, npq and lhc plants. In long-term treatment, the rate of DCMU (applied into substrate) uptake and interaction with light differed between wt and lhc plants. The results indicated that LHC-deficient A. thaliana mutants were more sensitive to photoinhibition than wt control. Supported by GACR52206097.
ER -
ŠTEPIGOVÁ, Jana, Kristýna VEČEŘOVÁ a Miloš BARTÁK. Does amount of light harvesting complexes play a role in the sensitivity of DCMU-treated Arabidopsis thaliana to high light stress? In \textit{7th International Conference of Ph.D. Students on Experimental Plant Biology, MZLU, Brno, Czech Republic, 30th June – 1st July 2009}. 2009. ISBN~978-80-7375-310-8.
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