Macrolide treatment failure in a case of secondary syphilis: a
novel A2059G mutation in the 23S rRNA gene of Treponema
pallidum subsp. pallidum.

J 2009

Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum.

MATĚJKOVÁ POSPÍŠILOVÁ, Petra, Magdalena FLASAROVÁ, Hana ZÁKOUCKÁ, Milan BOŘEK, Soňa KŘEMENOVÁ et. al.

Basic information

Original name

Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum.

Name in Czech

Selhání léčby sekundární syfilis makrolidovými antibiotiky: A2059G, nová mutace v genu pro 23S rRNA u Treponema pallidum subsp. pallidum.

Authors

MATĚJKOVÁ POSPÍŠILOVÁ, Petra (203 Czech Republic), Magdalena FLASAROVÁ (203 Czech Republic), Hana ZÁKOUCKÁ (203 Czech Republic), Milan BOŘEK (203 Czech Republic), Soňa KŘEMENOVÁ (203 Czech Republic), Petr ARENBERGER (203 Czech Republic), Vladana WOZNICOVÁ (203 Czech Republic), G.M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor)

Edition

Journal of Medical Microbiology, SOC GENERAL MICROBIOLOGY, 2009, 0022-2615

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.272

RIV identification code

RIV/00216224:14110/09:00029466

Organization unit

Faculty of Medicine

UT WoS

000266615900020

Keywords (in Czech)

benzathin-penicillin-G; azitromycinová rezistence; klinické vzorky; izolát; PCR

Keywords in English

penicillin-G benzathine; azithromycin resistance; clinical specimens; isolate; PCR

Změněno: 15/1/2010 13:31, Mgr. Marie Zobaníková, Ph.D.

Abstract

ORIG CZ

V originále

We report an occurrence of treatment failure after oral spiramycin therapy in a man with secondary syphilis and a reported penicillin and tetracycline allergy. Molecular detection revealed treponemal DNA in the blood of the patient and sequencing of the 23S rDNA identified an A to G transition at the gene position corresponding to position 2059 in the Escherichia coli 23S rRNA gene. The occurrence of this novel 23S rDNA mutation was examined among 7 rabbit-propagated syphilitic strains of Treponema pallidum and among 22 syphilis patient isolates from the Czech Republic. The prevalence of A2058G and A2059G mutations among clinical specimens was 18.2 and 18.2 %, respectively.

In Czech

Popsáno selhání léčby muže se sekundární syfilis po orálním podání spiramicinu. V krvi pacienta byla detekována treponemální DNA a sekvencování 23S rDNA odhalilo tranzici A na G v pozici, jež odpovídá pozici 2059 v genu pro 23S rRNA u druhu Escherichia coli.

Links

GA310/07/0321, research and development project

Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

NR8967, research and development project

Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministry of Health of the CR

Citovat

MATĚJKOVÁ POSPÍŠILOVÁ, Petra, Magdalena FLASAROVÁ, Hana ZÁKOUCKÁ, Milan BOŘEK, Soňa KŘEMENOVÁ, Petr ARENBERGER, Vladana WOZNICOVÁ, G.M. WEINSTOCK and David ŠMAJS. Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum. Journal of Medical Microbiology. SOC GENERAL MICROBIOLOGY, 2009, vol. 58, No 6, p. 832–836. ISSN 0022-2615.

@article{844664,
   author = {Matějková Pospíšilová, Petra and Flasarová, Magdalena and Zákoucká, Hana and Bořek, Milan and Křemenová, Soňa and Arenberger, Petr and Woznicová, Vladana and Weinstock, G.M. and Šmajs, David},
   article_number = {6},
   keywords = {penicillin-G benzathine; azithromycin resistance; clinical specimens; isolate; PCR},
   language = {eng},
   issn = {0022-2615},
   journal = {Journal of Medical Microbiology},
   title = {Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum.},
   url = {http://jmm.sgmjournals.org/cgi/reprint/58/6/832?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=Matejkova&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT},
   volume = {58},
   year = {2009}
}

TY  - JOUR
ID  - 844664
AU  - Matějková Pospíšilová, Petra - Flasarová, Magdalena - Zákoucká, Hana - Bořek, Milan - Křemenová, Soňa - Arenberger, Petr - Woznicová, Vladana - Weinstock, G.M. - Šmajs, David
PY  - 2009
TI  - Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum.
JF  - Journal of Medical Microbiology
VL  - 58
IS  - 6
SP  - 832–836
EP  - 832–836
PB  - SOC GENERAL MICROBIOLOGY
SN  - 00222615
KW  - penicillin-G benzathine
KW  - azithromycin resistance
KW  - clinical specimens
KW  - isolate
KW  - PCR
UR  - http://jmm.sgmjournals.org/cgi/reprint/58/6/832?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=Matejkova&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT
N2  - We report an occurrence of treatment failure after oral spiramycin therapy in a man with secondary syphilis and a reported penicillin and tetracycline allergy. Molecular detection revealed treponemal DNA in the blood of the patient and sequencing of the 23S rDNA identified an A to G transition at the gene position corresponding to position 2059 in the Escherichia coli 23S rRNA gene. The occurrence of this novel 23S rDNA mutation was examined among 7 rabbit-propagated syphilitic strains of Treponema pallidum and among 22 syphilis patient isolates from the Czech Republic. The prevalence of A2058G and A2059G mutations among clinical specimens was 18.2 and 18.2 %, respectively.
ER  -

MATĚJKOVÁ POSPÍŠILOVÁ, Petra, Magdalena FLASAROVÁ, Hana ZÁKOUCKÁ, Milan BOŘEK, Soňa KŘEMENOVÁ, Petr ARENBERGER, Vladana WOZNICOVÁ, G.M. WEINSTOCK and David ŠMAJS. Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in the 23S rRNA gene of Treponema pallidum subsp. pallidum. \textit{Journal of Medical Microbiology}. SOC GENERAL MICROBIOLOGY, 2009, vol.~58, No~6, p.~832–836. ISSN~0022-2615.

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