Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.

BENEŠOVÁ, Jana, Pavel DOBEŠ and Pavel HYRŠL. Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L. Bulletin of Insectology. Bologna, 2009, vol. 62, No 2, p. 237-243. ISSN 1721-8861.

Basic information

Original name

Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.

Name in Czech

Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.

Authors

BENEŠOVÁ, Jana (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic) and Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Bulletin of Insectology, Bologna, 2009, 1721-8861

---

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.448

RIV identification code

RIV/00216224:14310/09:00036599

Organization unit

Faculty of Science

UT WoS

000272023300017

Keywords (in Czech)

Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol.

Keywords in English

Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol.

Tags

International impact, Reviewed

---

Abstract

V originále

Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. PO activity changed gradually during development except during larval-pupal and pupal-adult metamorphosis, where marked increases in activity occurred. Comparable changes with slight time shifts were determined in cuticle after its homogenization. Intact cuticles showed only minimal PO activity without distinct developmental changes. Experiments using pyrogallol as substrate confirmed the temporary decrease of overall PO activity during pupal stage of development and the subsequent return to high PO levels in adults. Developmental changes in phenol-oxidizing activity are independent of sex in both pupal and adult stage of G. mellonella.

In Czech

Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. PO activity changed gradually during development except during larval-pupal and pupal-adult metamorphosis, where marked increases in activity occurred. Comparable changes with slight time shifts were determined in cuticle after its homogenization. Intact cuticles showed only minimal PO activity without distinct developmental changes. Experiments using pyrogallol as substrate confirmed the temporary decrease of overall PO activity during pupal stage of development and the subsequent return to high PO levels in adults. Developmental changes in phenol-oxidizing activity are independent of sex in both pupal and adult stage of G. mellonella.