HPLC Measurement of Thiamine and its Phosphorylated Forms in Whole Blood and Plasma: Thiamine Status Assessment

TOMANDLOVÁ, Marie, Josef TOMANDL and Kateřina KAŇKOVÁ. HPLC Measurement of Thiamine and its Phosphorylated Forms in Whole Blood and Plasma: Thiamine Status Assessment. In Euroanalysis. 2009.

Basic information

• Original name: HPLC Measurement of Thiamine and its Phosphorylated Forms in Whole Blood and Plasma: Thiamine Status Assessment
• Name in Czech: HPLC stanoveni thiaminu a jeho fosforylovaných forem v plné krvi a v plazmě: Stanovení thiaminového statusu
• Authors: TOMANDLOVÁ, Marie, Josef TOMANDL and Kateřina KAŇKOVÁ.
• Edition: Euroanalysis, 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Austria
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Organization unit: Faculty of Medicine
• Keywords (in Czech): thiamin; HPLC; plná krev; plazma
• Keywords in English: thiamine; HPLC; whole blood; plasma
• Tags: International impact

Abstract

A new gradient ion-paired HPLC utilising fluorescence detection for the simultaneous determination of thiamine and its mono- and diphosphate esters in human whole blood and plasma is developed. The method has been sufficient for the accurate, reliable and reproducible determination of thiamine status in healthy persons an also patients with diabetic nephropathy.

Abstract (in Czech)

Byla vyvinuta nová gradientová iontově-párová HPLC metoda, využívající fluorescenční detekci, pro současné stanovení thiaminu a jeho mono- a difosforečných esterů v lidské plné krvi a plazmě. Metoda je vhodná pro přesné, spolehlivé a reprodukovatelné určení stavu thiaminu u zdravých osob a rovněž u pacientů s diabetickou nefropatií.

Links

• LC06023, research and development project: Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

• NR9443, research and development project: Name: Genetická variabilita enzymů pentózového cyklu jako faktor modulující nástup a progresi diabetické nefropatie

Investor: Ministry of Health of the CR, Genetic variability of pentose phosphate pathway as a modulating factor of the onset and progression of diabetic nephropathy