| EISELLEOVÁ, Lívia, Kamil MATULKA, Vítězslav KŘÍŽ, Michaela KUNOVÁ, Zuzana SCHMIDTOVÁ, Jakub NERADIL, Boris TICHÝ, Dana DVOŘÁKOVÁ, Šárka POSPÍŠILOVÁ, Aleš HAMPL and Petr DVOŘÁK. A complex role for FGF-2 in self renewal, survival, and adhesion of human embryonic stem cells. Stem Cells. 2009, vol. 2009, No 27, p. 1847-1857, 10 pp. ISSN 1066-5099. |
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@article{845640,
author = {Eiselleová, Lívia and Matulka, Kamil and Kříž, Vítězslav and Kunová, Michaela and Schmidtová, Zuzana and Neradil, Jakub and Tichý, Boris and Dvořáková, Dana and Pospíšilová, Šárka and Hampl, Aleš and Dvořák, Petr},
article_number = {27},
keywords = {FGF-2; human ESCs; self renewal; cell survival; adhesion},
language = {eng},
issn = {1066-5099},
journal = {Stem Cells},
title = {A complex role for FGF-2 in self renewal, survival, and adhesion of human embryonic stem cells},
volume = {2009},
year = {2009}
}
TY - JOUR
ID - 845640
AU - Eiselleová, Lívia - Matulka, Kamil - Kříž, Vítězslav - Kunová, Michaela - Schmidtová, Zuzana - Neradil, Jakub - Tichý, Boris - Dvořáková, Dana - Pospíšilová, Šárka - Hampl, Aleš - Dvořák, Petr
PY - 2009
TI - A complex role for FGF-2 in self renewal, survival, and adhesion of human embryonic stem cells
JF - Stem Cells
VL - 2009
IS - 27
SP - 1847-1857
EP - 1847-1857
SN - 10665099
KW - FGF-2
KW - human ESCs
KW - self renewal
KW - cell survival
KW - adhesion
N2 - The transcription program that is responsible for the pluripotency of human ESCs (hESCs) is believed to be comaintained by exogenous fibroblast growth factor-2 (FGF-2), which activates FGF receptors (FGFRs) and stimulates the mitogen-activated protein kinase (MAPK) pathway. This mechanism is further complicated by intracrine FGF signals.Here we show that, in undifferentiated hESCs, exogenous FGF-2 and inhibition of autocrine FGF signaling stimulated the expression of stem cell genes while suppressing cell death and apoptosis genes. Thus, exogenous FGF-2 reinforced the pluripotency maintenance program of intracrine FGF-2 signaling. Consistent with this hypothesis, expression of endogenous FGF-2 decreased during hESC differentiation and FGF-2 knockdown-induced hESC differentiation. In addition, FGF-2 signaling via FGFR2 activated MAPK kinase/extracellular signal-regulated kinase and AKT kinases, protected hESC from stress-induced cell death, and increased hESC adhesion and cloning efficiency.
ER -
EISELLEOVÁ, Lívia, Kamil MATULKA, Vítězslav KŘÍŽ, Michaela KUNOVÁ, Zuzana SCHMIDTOVÁ, Jakub NERADIL, Boris TICHÝ, Dana DVOŘÁKOVÁ, Šárka POSPÍŠILOVÁ, Aleš HAMPL and Petr DVOŘÁK. A complex role for FGF-2 in self renewal, survival, and adhesion of human embryonic stem cells. \textit{Stem Cells}. 2009, vol.~2009, No~27, p.~1847-1857, 10 pp. ISSN~1066-5099.
|
