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@article{845823, author = {Buňková, Leona and Buňka, František and Hlobilová, Michaela and Vaňátková, Zuzana and Nováková, Dana and Dráb, Vladimír}, article_number = {229}, keywords = {tyramine production; Lactobacillus; Lactococcus; Streptococcus}, language = {eng}, issn = {1438-2377}, journal = {European Food Research and Technology}, title = {Tyramine production of technological important strains of Lactobacillus, Lactococcus and Streptococcus}, volume = {2009}, year = {2009} }
TY - JOUR ID - 845823 AU - Buňková, Leona - Buňka, František - Hlobilová, Michaela - Vaňátková, Zuzana - Nováková, Dana - Dráb, Vladimír PY - 2009 TI - Tyramine production of technological important strains of Lactobacillus, Lactococcus and Streptococcus JF - European Food Research and Technology VL - 2009 IS - 229 SP - 553-558 EP - 553-558 SN - 14382377 KW - tyramine production KW - Lactobacillus KW - Lactococcus KW - Streptococcus N2 - The aim of this paper was to study the biogenic amines (histamine, tyramine, putrescine, cadaverine, agmatine, spermine and spermidine) production of selected technological important lactic acid bacteria (strains of the genera Lactococcus, Lactobacillus and Streptococcus). Three methods (ion-exchange chromatography (IEC), PCR and cultivation method with pH indicator) were used. Within the 39 strains of lactic acid bacteria tested, the production of tyramine (formed by tyrosine decarboxylase) was detected in eight strains (3 strains of Lactococcus lactis subsp. lactis, three strains of Lactococcus lactis subsp. cremoris, 1 strain of Streptococcus thermophilus and 1 strain of Lactobacillus delbrueckii subsp. bulgaricus). The other tested biogenic amines were not detected. Cultivation in decarboxylation broth seems to be the least accurate method for the detection of biogenic amines due to enhanced risk of false-positive reactions. Therefore, in order to detect bacteria producing biogenic amines, the combination of PCR and chromatographic methods (e.g. IEC) can be recommended. ER -
BUŇKOVÁ, Leona, František BUŇKA, Michaela HLOBILOVÁ, Zuzana VAŇÁTKOVÁ, Dana NOVÁKOVÁ and Vladimír DRÁB. Tyramine production of technological important strains of Lactobacillus, Lactococcus and Streptococcus. \textit{European Food Research and Technology}. 2009, vol.~2009, No~229, p.~553-558. ISSN~1438-2377.
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