Detailed Information on Publication Record
2009
miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I
HÉŽOVÁ, Renata, Ondřej SLABÝ, Petra FALTEJSKOVÁ, Zuzana MIKULKOVÁ, Ivana BUREŠOVÁ et. al.Basic information
Original name
miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I
Name in Czech
miRNA expresní profil regulačních T lymfocytů u pacientů s diabetes mellitus typu I
Authors
HÉŽOVÁ, Renata, Ondřej SLABÝ, Petra FALTEJSKOVÁ, Zuzana MIKULKOVÁ, Ivana BUREŠOVÁ, J. HODEK, J. OVESNÁ and Jaroslav MICHÁLEK
Edition
The 4th Asian Congress on Autoimmunity, Singapore, 2009
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
30202 Endocrinology and metabolism
Country of publisher
Singapore
Confidentiality degree
není předmětem státního či obchodního tajemství
Organization unit
Faculty of Medicine
Keywords (in Czech)
diabetes; regulační T lymfocyty; miRNA
Keywords in English
Diabetes mellitus; regulatory T cells; miRNA
Změněno: 5/9/2012 16:18, Mgr. Petra Vychytilová, Ph.D.
V originále
MicroRNA (miRNA) are small non-coding RNA molecules, that have a potential to regulate the expression of many genes. Type I diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic beta cells by cytotoxic T cells. Regulatory T cells (Treg) are specialised lymphocytes preventing from development of autoimmune disorders. Study on transgenic mouse with conditional Dicer knockout Treg revealed that this Treg cell-specific miRNA ablation results in the loss of suppressor function and development of fatal systemic autoimmune disease in vivo. Therefore we can assume, that the functional defect of Treg in T1D patients can be caused by different expression miRNA profile in these cells. The aim of our study was to establish expression profile of miRNA in patients with T1D and compare it with healthy donors. Six diabetic patients and 6 healthy volunteers provided peripheral blood for flow cytometry analyses and separation of Treg (CD3+, CD4+, CD25+, CD127-) and non-Treg cells (CD3+, CD4+, CD25-). Total RNA rich in small RNAs was isolated and used for TaqMan Human MicroRNA Array that analysed expression of 384 miRNA in Treg and non-Treg cells. The data were subsequently processed by HCL, SAM analysis. Different miRNA expression patterns were identified in Treg and non-Treg populations as well as in Treg cells between T1D and healthy individuals. We aimed to identify miRNA specific in pathogenesis of diabetes mellitus which could be used in the future as therapeutic targets of diabetic patients.
In Czech
Cílem prezentované studie bylo získat expresní profil miRNA u pacientů s T1D a porovnat jej s profilem zdravých dárců.
Links
NR9355, research and development project |
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2B08066, research and development project |
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