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@proceedings{847940, author = {Babíčková, Karolina and Hyršl, Pavel and Tolarová, Soňa}, booktitle = {3rd EVIW European Veterinary Immunology Workshop}, keywords = {bacteriolytic activity; complement; lysozyme; temperature inactivation; bioluminescence; recombinant bacteria}, language = {eng}, title = {Antibacterial activity of lysozyme and complement in canine blood sera}, year = {2009} }
TY - CONF ID - 847940 AU - Babíčková, Karolina - Hyršl, Pavel - Tolarová, Soňa PY - 2009 TI - Antibacterial activity of lysozyme and complement in canine blood sera KW - bacteriolytic activity KW - complement KW - lysozyme KW - temperature inactivation KW - bioluminescence KW - recombinant bacteria N2 - The aim of this work was to analyse total bacteriolytic activity of complement and lysozyme concentration in 235 samples of canine blood sera. Method based on the bioluminescence of recombinant bacteria Escherichia coli was used for complement activity measurement. Lysozyme concentration was determined by radial diffusion in agarose gels. Temperature of inactivation of complement cascade was measured in few selected samples and was set to 49-50 Celsius degrees. Differences of complement activity and lysozyme concentration between age and weight categories of male dogs and bitches individually were compared. Statistical differences of complement activity between indvidual groups of age cathegories and also of weight cathegories of bitches were found. Statistical differences of lysozyme concentration between indvidual groups of age cathegories of male dogs were also found. No statistically significant correlation between complement activity and lysozyme concentration was determined. ER -
BABÍČKOVÁ, Karolina, Pavel HYRŠL a Soňa TOLAROVÁ. Antibacterial activity of lysozyme and complement in canine blood sera. In \textit{3rd EVIW European Veterinary Immunology Workshop}. 2009.
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