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@article{848202,
author = {Falk, Martin and Lukášová, Emilie and Kozubek, Stanislav},
article_location = {Amsterdam},
article_number = {12},
doi = {http://dx.doi.org/10.1016/j.bbamcr.2008.07.010},
keywords = {Chromatin structure; DNA damage; Double-strand break (DSB); DNA repair; Experimentally changed radiosensitivity; Apoptosis},
language = {eng},
issn = {0167-4889},
journal = {Biochimica et biophysica acta : Molecular Cell Research},
title = {Chromatin structure influences the sensitivity of DNA to gamma-radiation},
volume = {1783},
year = {2008}
}
TY - JOUR
ID - 848202
AU - Falk, Martin - Lukášová, Emilie - Kozubek, Stanislav
PY - 2008
TI - Chromatin structure influences the sensitivity of DNA to gamma-radiation
JF - Biochimica et biophysica acta : Molecular Cell Research
VL - 1783
IS - 12
SP - 2398-2414
EP - 2398-2414
PB - Elsevier
SN - 01674889
KW - Chromatin structure
KW - DNA damage
KW - Double-strand break (DSB)
KW - DNA repair
KW - Experimentally changed radiosensitivity
KW - Apoptosis
N2 - For the first time, DNA double-strand breaks (DSBs) were directly visualized in functionally and structurally different chromatin domains of human cells. The results show that genetically inactive condensed chromatin is much less susceptible to DSB induction by gamma-rays than expressed, decondensed domains. Higher sensitivity of open chromatin for DNA damage was accompanied by more efficient DSB repair. These findings follow from comparing DSB induction and repair in two 11 Mbp-long chromatin regions, one with clusters of highly expressed genes and the other, gene-poor, containing mainly genes having only low transcriptional activity. The same conclusions result from experiments with whole chromosome territories, differing in gene density and consequently in chromatin condensation. It follows from our further results that this lower sensitivity of DNA to the damage by ionizing radiation in heterochromatin is not caused by the simple chromatin condensation but very probably by the presence of a higher amount of proteins compared to genetically active and decondensed chromatin. In addition, our results show that some agents potentially used for cell killing in cancer therapy (TSA, hypotonic and hypertonic) influence cell survival of irradiated cells via changes in chromatin structure and efficiency of DSB repair in different ways. (C) 2008 Elsevier B.V. All rights reserved.
ER -
FALK, Martin, Emilie LUKÁŠOVÁ and Stanislav KOZUBEK. Chromatin structure influences the sensitivity of DNA to gamma-radiation. \textit{Biochimica et biophysica acta : Molecular Cell Research}. Amsterdam: Elsevier, 2008, vol.~1783, No~12, p.~2398-2414. ISSN~0167-4889. Available from: https://dx.doi.org/10.1016/j.bbamcr.2008.07.010.
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