| FALK, Martin, Emilie LUKÁŠOVÁ, Barbora GABRIELOVÁ, Vladan ONDŘEJ and Stanislav KOZUBEK. Local changes of higher-order chromatin structure during DSB-repair. JOURNAL OF PHYSICS CONFERENCE SERIES. BRISTOL: IOP PUBLISHING LTD, England, 2008, -, No 101, p. 12018-12035. ISSN 1742-6588. |
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@article{848213,
author = {Falk, Martin and Lukášová, Emilie and Gabrielová, Barbora and Ondřej, Vladan and Kozubek, Stanislav},
article_location = {BRISTOL},
article_number = {101},
keywords = {DOUBLE-STRAND BREAKS; HIGH-RESOLUTION CYTOMETRY; DNA-DAMAGE; CHROMOSOME DOMAINS; ACTIVE GENES; NUCLEI; SITES; PROTEINS; CELLS; TRANSCRIPTION},
language = {eng},
issn = {1742-6588},
journal = {JOURNAL OF PHYSICS CONFERENCE SERIES},
title = {Local changes of higher-order chromatin structure during DSB-repair},
volume = {-},
year = {2008}
}
TY - JOUR
ID - 848213
AU - Falk, Martin - Lukášová, Emilie - Gabrielová, Barbora - Ondřej, Vladan - Kozubek, Stanislav
PY - 2008
TI - Local changes of higher-order chromatin structure during DSB-repair
JF - JOURNAL OF PHYSICS CONFERENCE SERIES
VL - -
IS - 101
SP - 12018
EP - 12018
PB - IOP PUBLISHING LTD, England
SN - 17426588
KW - DOUBLE-STRAND BREAKS
KW - HIGH-RESOLUTION CYTOMETRY
KW - DNA-DAMAGE
KW - CHROMOSOME DOMAINS
KW - ACTIVE GENES
KW - NUCLEI
KW - SITES
KW - PROTEINS
KW - CELLS
KW - TRANSCRIPTION
N2 - We show that double-strand breaks (DSBs) induced in DNA of human cells by gamma-radiation arise mainly in active, gene-rich, decondensed chromatin. We demonstrate that DSBs show limited movement in living cells, occasionally resulting in their permanent clustering, which poses a risk of incorrect DNA rejoining. In addition, some DSBs remain unrepaired for several days after irradiation, forming lesions repairable only with difficulty which are hazardous for genome stability. These late DSBs colocalize with heterochromatin markers (dimethylated histone H3 at lysine 9, HP1 and CENP-A proteins), despite the low density of the surrounding chromatin. This indicates that there is epigenetic silencing of loci close to unrepaired DSBs and/or stabilization of damaged decondensed chromatin loops during repair and post-repair reconstitution of chromatin structure.
ER -
FALK, Martin, Emilie LUKÁŠOVÁ, Barbora GABRIELOVÁ, Vladan ONDŘEJ and Stanislav KOZUBEK. Local changes of higher-order chromatin structure during DSB-repair. \textit{JOURNAL OF PHYSICS CONFERENCE SERIES}. BRISTOL: IOP PUBLISHING LTD, England, 2008, -, No~101, p.~12018-12035. ISSN~1742-6588.
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