STIXOVÁ, Lenka, Jiřina PROCHÁZKOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ and Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. Mol Cell Biochem. 2009, vol. 330, 1-2, 10 pp. ISSN 0300-8177.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway
Authors STIXOVÁ, Lenka (203 Czech Republic, guarantor), Jiřina PROCHÁZKOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic), Jiřina HOFMANOVÁ (203 Czech Republic) and Alois KOZUBÍK (203 Czech Republic).
Edition Mol Cell Biochem, 2009, 0300-8177.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.896
RIV identification code RIV/00216224:14310/09:00028603
Organization unit Faculty of Science
UT WoS 000270687500025
Keywords in English Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;
Tags International impact, Reviewed
Changed by Changed by: Mgr. Jiřina Medalová, Ph.D., učo 176525. Changed: 25/3/2010 11:43.
Abstract
The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation.
PrintDisplayed: 3/10/2024 13:22