5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin
D3 induced monocytic differentiation by activating p38 MAPK
pathway

J 2009

5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway

STIXOVÁ, Lenka, Jiřina PROCHÁZKOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ, Alois KOZUBÍK et. al.

Basic information

Original name

5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway

Authors

STIXOVÁ, Lenka (203 Czech Republic, guarantor), Jiřina PROCHÁZKOVÁ (203 Czech Republic), Karel SOUČEK (203 Czech Republic), Jiřina HOFMANOVÁ (203 Czech Republic) and Alois KOZUBÍK (203 Czech Republic)

Edition

Mol Cell Biochem, 2009, 0300-8177

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.896

RIV identification code

RIV/00216224:14310/09:00028603

Organization unit

Faculty of Science

UT WoS

000270687500025

Keywords in English

Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;

Abstract

V originále

The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation.

Citovat

STIXOVÁ, Lenka, Jiřina PROCHÁZKOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ and Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. Mol Cell Biochem. 2009, vol. 330, 1-2, 10 pp. ISSN 0300-8177.

@article{849022,
   author = {Stixová, Lenka and Procházková, Jiřina and Souček, Karel and Hofmanová, Jiřina and Kozubík, Alois},
   article_number = {1-2},
   keywords = {Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;},
   language = {eng},
   issn = {0300-8177},
   journal = {Mol Cell Biochem},
   title = {5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway},
   volume = {330},
   year = {2009}
}

TY  - JOUR
ID  - 849022
AU  - Stixová, Lenka - Procházková, Jiřina - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois
PY  - 2009
TI  - 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway
JF  - Mol Cell Biochem
VL  - 330
IS  - 1-2
SN  - 03008177
KW  - Differentiation
KW  - 1alpha25 dihydroxyvitamin D3
KW  - 5 lipoxygenase inhibitors
KW  - p38 MAPK;
N2  - The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation.
ER  -

STIXOVÁ, Lenka, Jiřina PROCHÁZKOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ and Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. \textit{Mol Cell Biochem}. 2009, vol.~330, 1-2, 10 pp. ISSN~0300-8177.

Detailed Information on Publication Record