MATEJKOVÁ, Michaela, Jitka ŽÍDKOVÁ, Lukáš ŽÍDEK, Michaela WIMMEROVÁ, Josef CHMELÍK and Vladimír SKLENÁŘ. Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry. Journal of Agricultural and Food Chemistry. WASHINGTON: AMER CHEMICAL SOC, 2009, vol. 57, No 18, p. 8444-8452. ISSN 0021-8561.
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Basic information
Original name Investigation of Thermal Denaturation of Barley Nonspecific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry
Name in Czech Zkoumání teplotních denaturací nespecifického lipid transfer proteinu 1 (ns-LTP1b) pomocí nukleární magnetické resonance a diferenciální skenovací kalorimetrie
Authors MATEJKOVÁ, Michaela (703 Slovakia, belonging to the institution), Jitka ŽÍDKOVÁ (203 Czech Republic, belonging to the institution), Lukáš ŽÍDEK (203 Czech Republic, guarantor, belonging to the institution), Michaela WIMMEROVÁ (203 Czech Republic, belonging to the institution), Josef CHMELÍK (203 Czech Republic) and Vladimír SKLENÁŘ (203 Czech Republic, belonging to the institution).
Edition Journal of Agricultural and Food Chemistry, WASHINGTON, AMER CHEMICAL SOC, 2009, 0021-8561.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 21101 Food and beverages
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.469
RIV identification code RIV/00216224:14310/09:00036779
Organization unit Faculty of Science
UT WoS 000269747500053
Keywords in English Non-specific lipid transfer protein 1; barley grain; thermaldenaturation; nuclear magnetic resonance; differential scanningcalorimetry
Tags International impact, Reviewed
Changed by Changed by: prof. Mgr. Lukáš Žídek, Ph.D., učo 38990. Changed: 9/3/2015 05:39.
Abstract
Process of thermal denaturation of covalently modified form of barley grain non-specific lipid transfer protein 1b (ns-LTP1b) was investigated by nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) up to 115\,$^\circ$C. The denaturation was found to be irreversible and highly cooperative. A method of numerical quantitative analysis allowing to fit the NMR data to a transition-state model without further simplification was developed. Based on the obtained values of transition state enthalpy and entropy, rate of denaturation was calculated as a simple measure of protein stability at various temperatures. Effect of disulfide bond reduction on thermal denaturation of ns-LTP1b was studied and discussed in the context of quality control of barley products during storage and processing.
Abstract (in Czech)
Process of thermal denaturation of covalently modified form of barley grain non-specific lipid transfer protein 1b (ns-LTP1b) was investigated by nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) up to 115\,$^\circ$C. The denaturation was found to be irreversible and highly cooperative. A method of numerical quantitative analysis allowing to fit the NMR data to a transition-state model without further simplification was developed. Based on the obtained values of transition state enthalpy and entropy, rate of denaturation was calculated as a simple measure of protein stability at various temperatures. Effect of disulfide bond reduction on thermal denaturation of ns-LTP1b was studied and discussed in the context of quality control of barley products during storage and processing.
Links
LC06030, research and development projectName: Biomolekulární centrum
Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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